Establishment of HPLC Fingerprint of Different Polar Parts of Zhuang Medicine Calonyction muricatum and Spectrum-effect Relationship of Its Analgesic and Anti-inflammatory Effects
- VernacularTitle:壮药华佗豆不同极性部位HPLC指纹图谱的建立及其镇痛抗炎作用的谱效关系
- Author:
Jing LIN
1
,
2
;
Jie LIANG
1
,
3
,
4
;
Chunyan HUANG
5
;
Jinyu WEI
1
;
Dongfang HUANG
1
;
Jue HU
1
;
Jinli QI
1
;
Huihua CHEN
1
Author Information
1. School of Pharmacy，Guangxi University of TCM，Nanning 530200，China
2. Dept. of Pharmacy，Guangxi Agricultural Vocational College，Nanning 530007，China
3. Guangxi Zhuang Yao Medicine Center of Engineering and Technology，Nanning 530200，China
4. Guangxi Superior Chinese Patent Medicine and Ethnic Medicine Development Engineering Technology Center，Nanning 530200，China
5. Qin zhou Inspection and Test Institute，Guangxi Qin zhou 535000，China
- Publication Type:Journal Article
- Keywords:
Calonyction muricatum;
Analgesic and anti-inflammatory;
Fingerprint;
Spectrum-effect relationship
- From:
China Pharmacy
2021;32(17):2079-2084
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE：To establish HPLC fingerprints of different polar parts of Zhuang medicine Calonyction muricatum ， and to study its spectrum-effect relationship with analgesic and anti-inflammatory effects. METHODS ：The total part ，ethyl acetate part，n-butanol part and water part of C. muricatum were prepared. HPLC fingerprints of different polar parts were established by HPLC method combined with the Similarity Evaluation System of TCM Chromatogramtic Fingerprint （2012A），and the common peaks were identified. Using writhing times and ear swelling degree in mice as analgesic and anti-inflammatory indexes ，analgesic and anti-inflammatory activity of different polar parts of C. muricatum were investigated. The correlation of the common peaks of HPLC fingerprint with analgesic and anti-inflammatory indexes was analyzed by grey correlation analysis ，bivariate correlation analysis and partial least square （PLS） method. RESULTS ： There were 11 common peaks for the different polar parts of C. muricatum ，and 5 components were identified by reference comparison，i.e. neochlorogenic acid （peak 3），chlorogenic acid （peak 5）， cryptochlorogenic acid （peak 6）， isochlorogenic acid A （peak 10），isochlorogenic acid C （peak 11）. The grey correlation analysis showed that the correlation between all common peaks and analgesic and anti- inflammatory effects were greater than 0.6 （except the correlation between peak 6 and analgesic effects ），showing correlation relationship ；the correlation of peaks 3，7 and 10 with analgesic and anti-inflammatory effects were all greater than 0.8，which was highly related. Bivariate correlation analysis showed that the correlation of peak 1，3，4，7，9，10，11 with analgesic and anti-inflammatory effects were all greater than 0.6，showing correlation relationship. PLS method showed that peaks 1，3，4，7，9，10，11 contributed greatly to playing an analgesic and anti-inflammatory role. CONCLUSIONS ：HPLC fingerprints of different polar parts of C. muricatum is established and five common peak components were identified. Neochlorogenic acid ，isochlorogenic acid A ，isochlorogenic acid C and chemical components represented by peaks 1，4，7，9 may be the pharmacodynamic substances of C. muricatum to exert analgesic and anti-inflammatory effects.
