Effects and mechanisms of microRNA-23b on neuronal apoptosis induced by Aβ25-35
10.11665/j.issn.1000-5048.20210411
- VernacularTitle:microRNA-23b对Aβ25-35导致神经细胞凋亡的作用及机制研究
- Author:
Kemeng PAN
1
;
Song CHEN
;
Xiangdong GAO
;
Wenbing YAO
Author Information
1. 中国药科大学生命科学与技术学院 江苏省生物药物成药性研究重点实验室
- Publication Type:Journal Article
- Keywords:
microRNA-23b;
Alzheimer′s disease;
β-amyloid;
apoptosis;
autophagy
- From:
Journal of China Pharmaceutical University
2021;52(4):480-486
- CountryChina
- Language:Chinese
-
Abstract:
In this study, the effects and mechanisms of miR-23b in the AD cell model were explored. Aβ25-35 was used to induce neuronal injury model, and cell viabilities were detected by MTT assay. The effect of miR-23b on the apoptotic levels of Aβ25-35-induced SH-SY5Y cells was analyzed using Annexin V-FITC/PI detection kit. The effect of miR-23b on the mitochondrial membrane potential of Aβ25-35-induced SH-SY5Y cells was examined using JC-1 fluorescent probe. The levels of cell apoptosis-related proteins and autophagy-related proteins were detected by Western blot. The results showed that miR-23b could alleviate the apoptosis and the abnormal mitochondrial membrane potential in SH-SY5Y cells induced by Aβ25-35.This study suggested that miR-23b may attenuate Aβ25-35-induced neuronal apoptosis by regulating apoptosis and autophagy-related pathway.
