Effect of sanguinarine combined with cisplatin on the apoptosis of bladder cancer EJ cells
10.11665/j.issn.1000-5048.20210409
- VernacularTitle:血根碱与顺铂联用对膀胱癌EJ细胞凋亡的影响
- Author:
Da SHI
1
;
Qifang LEI
;
Tao TAO
;
Wenlong MA
;
Shuixian YE
;
Guangzhi LI
;
Haiyan SUN
;
Song WU
Author Information
1. 安徽理工大学医学院
- Publication Type:Journal Article
- Keywords:
sanguinarine;
cisplatin;
bladder cancer;
apoptosis;
combination medication
- From:
Journal of China Pharmaceutical University
2021;52(4):463-471
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the effects of sanguinarine (Sang) combined with cisplatin (Cis) in accelerating the apoptosis of bladder cancer EJ cells, CCK-8 method was used to detect the proliferation of bladder cancer EJ cells treated with different concentrations of Sang with the IC50 values calculated. Annexin V FITC/PI method was used to detect cell apoptosis in the control group, Sang group, Cis group and the combination group. Flow cytometer was used to detect cell cycle arrested. Western blot was used to detect the influence of Bcl-2 expression in the control group, Sang group, Cis group and the combination group. Nude mouse subcutaneous tumor model was constructed to verify that the combination group could accelerate the apoptosis of bladder cancer EJ cells and reduce the side-effects on mice. The safety of the Sang was evaluated by HE staining of vital organs in mice. In vitro, Sang could significantly inhibit the proliferation of EJ cells. Compared with the control group, the number of apoptosis EJ cells in the combination group was significantly increased (P < 0.05), and more cells were arrested in G2/M phase. The expression of Bcl-2 was significantly down-regulated in the combination group (P <0.001). In vivo, compared with the control group, the tumor growth was significantly slower, and a large number of apoptotic cells were inspected (P < 0.05) of the combination group. The side effects of cisplatin were reduced in the combination group. Sang has high biosafety and little side effect. Combined Sang and Cis can increase cell cycle G2/M block, down-regulate Bcl-2 expression, promote cell apoptosis and inhibit tumor growth.
