Secondary metabolites of the endophytic fungus Myrothecium sp. from Pinellia ternata
10.11665/j.issn.1000-5048.20210405
- VernacularTitle:一株植物内生真菌漆斑菌次级代谢产物的研究
- Author:
Juping YU
1
;
Minghua YANG
;
Xiaorong HE
Author Information
1. 中国药科大学工学院
- Publication Type:Journal Article
- Keywords:
Pinellia ternata;
endophytic fungi;
Myrothecium sp.;
verrucarin;
roridin
- From:
Journal of China Pharmaceutical University
2021;52(4):431-437
- CountryChina
- Language:English
-
Abstract:
Using a series of purification methods including silica gel， Sephadex LH-20 and preparative high performance liquid chromatography， secondary metabolites of Myrothecium sp. were purified from the ethyl acetate extract of the solid fermentation product. Based on structure characterization and investigation on the physical and chemical properties a， twelve monomeric compounds were identified as 3''-hydroxyverrucarin A (1), verrucarin A (2), verrucarin L acetate (3), verrucarin J (4), verrucarin K (5), roridin A (6), roridin D (7), roridin H (8), roridin J (9), verrol 4-acetate (10), (3S, 3aS, 6α, 6aR)-dihydrosporothrioride (11) and 4,6-dihydroxy-1(3H)-isobenzofuranone (12). Compounds 1, 5 and 9 -12 were isolated from Myrothecium sp. for the first time.Compounds 2, 3, 4, 7 and 8 exhibited strong inhibitory effects on Candida albicans, with a minimal inhibitory concentration （MIC50） of 0.318, 0.218, 0.047, 0.569 and 0.558 μg/mL, respectively.
