Comparison of MALDI-TOF mass spectrometry with phenotypic methods for identification and characterization of Staphylococcus aureus causing mastitis
https://doi.org/10.47665/tb.38.2.032
- Author:
Alharbi, A.
1
,
2
;
Al-Dubaib, M.
1
;
Elhassan M.A. Saeed
1
,
3
;
Elbehiry, A.
4
,
5
Author Information
1. Department of Veterinary Medicine, College of Agriculture and Veterinary Medicine, Qassim University, Saudi Arabia&
2. Ministry of Environment, Water and Agriculture, Saudi Arabia
3. Department of Microbiology, Faculty of Veterinary Medicine, Khartoum University
4. Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, University of Sadat City, Egypt&
5. Department of Public Health, College of Public Health and Health Informatics, Al Bukayriyah, Qassim University
- Publication Type:Journal Article
- Keywords:
S. aureus mastitis;
MRSA;
single peak;
PCA;
antimicrobial resistance
- From:Tropical Biomedicine
2021;38(No.2):9-24
- CountryMalaysia
- Language:English
-
Abstract:
The emergence of methicillin-resistant Staphylococcus aureus (MRSA) infection is one of the
greatest threats to both animal and human health. Our investigation was aimed to identify
and differentiate between MRSA and methicillin-sensitive Staphylococcus aureus (MSSA)
recovered from mastitic milk using MALDI-TOF mass spectrometry compared with phenotypic
methods and studying their susceptibility to various antibiotics. Four hundred milk samples
from mastitic animals (cows, sheep, goats, and dromedary camels) were investigated.
Phenotypic identification of S. aureus was made through MASTASAPH Latex test, STAPH ID 32,
and Vitek 2 system. The proteomic characterization of S. aureus was done by MBT. The Kirby
Bauer method was accomplished to detect the resistance of S. aureus strains to antibiotics.
The results of the MASTASAPH Latex test, revealed that 54 (46%) were recognized as S. aureus.
All S. aureus isolates were identified by MBT with a score of more or equal 2.00. Several peaks
were identified in the mass of 4590 Da, 4863 Da, and 4938 Da for MSSA and in the mass of
2636 Da and 3009 Da for MRSA. The MSP dendrogram demonstrated that the S. aureus isolates
were classified into one group with a distance level of less or equal 400. The percentage of
S. aureus resistance against carbenicillin, erythromycin and kanamycin was 94.4%, 38.88%,
and 33.33%, respectively. In conclusion, S. aureus bacteria are among the key triggers for
mastitis in Saudi Arabia. MBT is reported to be not only the rapid tool to identify S. aureus but
also able to discriminate MRSA from MSSA.
- Full text:8.2021my1232.pdf
