Establishment of UPLC Fingerprint of Hefu Zhiyang Decoction and Content Determination of 8 Components
- VernacularTitle:和肤止痒方的UPLC指纹图谱建立及8种成分的含量测定
- Author:
Lijuan LIU
1
;
Ya ZHAO
1
;
Yayun WU
1
;
Ruizhi ZHAO
1
;
Chuanjian LU
2
Author Information
1. Innovative Team for Modern Interpretation of TCM Property Theory，the Second Affiliated Hospital of Guangzhou University of TCM，Guangzhou 510006，China
2. Dermatological Department，the Second Affiliated Hospital of Guangzhou University of TCM，Guangzhou 510120，China
- Publication Type:Journal Article
- Keywords:
Hefu zhiyang decoction;
UPLC;
Fingerprint
- From:
China Pharmacy
2021;32(16):1982-1987
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE：To establish th e fingerp rint and c onduct cluster analysis of Hefu zhiyang decoction （HFZYD），and establish the method for content determination of 8 components，so as to provide reference for the quality control of HFZYD. METHODS：UPLC method was adopted. The determination was performed on ACQUITY UPLC BEH C 18 column with mobile phase consisted of acetonitrile- 0.1％ glacial acetic acid solution （gradient elution ）at the flow rate of 0.25 mL/min. The column temperature maintained at 30 ℃，and detection wavelength was 236 nm. The sample size was 5 μL. The fingerprint of 10 batches of HFZYD was established with Similarity Evaluation System of TCM Chromatographic Fingerprints （2012 edition）. Compared with substance control ，the common peaks were identified. The similarity evaluation was conducted. SPSS 21.0 software was used to conduct cluster analysis of 10 batches of samples. The contents of 8 components such as gallic acid in 10 batches of samples were determined by above UPLC. RESULTS ：There were 34 common peaks in the UPLC fingerprint of 10 batches of HFZYD ， identified as peak 1 was gallic acid ，peak 10 was ferulic acid ，peak 13 was astilbin ，peak 23 was paeonol ，peak 28 was dictamnine，peak 31 was obacunone ，peak 32 was fraxinellone ，and peak 34 was osthole. Its similarity with the control fingerprint was 0.923-0.979. Among 10 batches of samples ，S1，S2，S5，S6，S7，S8 and S 10 were clustered into one category ，and S 3，S4 and S 9 were clustered into one category. The average contents of above 8 components were 0.596-0.714，0.262-0.321，7.647-9.859， 0.113-0.644，0.170-0.202，0.854-1.281，0.631-0.857，3.243-3.548 mg/g，respectively. CONCLUSIONS ：UPLC fingerprint and the method for content determination of 8 components in HFZYD are established successfully.
