Simultaneous Determination of 5 Components in the Classical Formula Huaihua San by HPLC Wavelength Switching Method
- VernacularTitle:HPLC波长切换法同时测定经典名方槐花散中5种成分的含量
- Author:
Zedong XIANG
1
;
Qing XUE
1
;
Haitao YU
2
;
Zhiqiang SUN
2
;
Zhen LI
1
;
Juyan LIU
3
;
Jiayu ZHANG
4
;
Peng GAO
1
;
Long DAI
4
Author Information
1. College of Pharmacy，Shandong University of TCM，Jinan 250355，China
2. Shandong Yuze Institute of Pharmaceutical and Health Industry Technology，Shandong Dezhou 251200，China
3. Technology and Quality Department，Guangzhou Pharmaceutical Group Co.，Ltd，Guangzhou 510130，China
4. School of Pharmacy，Binzhou M edical University，Shandong Yantai 264003，China
- Publication Type:Journal Article
- Keywords:
Huaihua san;
HPLC wavelength switching method;
Rutin;
Naringin;
Neohesperidin;
Quercetin;
Pulegone
- From:
China Pharmacy
2021;32(16):1964-1968
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE：To develop a method for simultaneous determination of 5 components in classical formula Huaihua san，including rutin ，naringin，neohesperidin，quercetin and pulegone. METHODS ：HPLC wavelength switching method was adopted. The determination was performed on Cosmosil C 18 column with mobile phase consisted of acetonitrile- 0.05％ phosphoric acid solution （gradient elution ）at the flow rate of 1.0 mL/min. The detection wavelengths were set at 257 nm for rutin ，283 nm for naringin and neohesperidin ，254 nm for quercetin ，252 nm for pulegone ，respectively. The column temperature was set at 30 ℃， and sample size was 10 μL. RESULTS：The linear range was 21.7-2 170 μg/mL for rutin，46-4 600 μg/mL for naringin，22.3- 2 230 μg/mL for neohesperidin，0.96-96 μg/mL for quercetin，2.7-270 μg/mL for pulegone（all r＞0.999），respectively. RSDs of precision，stability（24 h）and reproducibility tests were all lower than 2％（n＝6）. Average recoveries were 100.70％，99.31％， 101.10％，100.03％ and 99.63％（all RSD ＜2％，n＝9）. Among 3 batches of Huaihua san samples ，the contents of above 5 components were 20.055-22.615，25.557-27.806，11.428-13.250，0.350-0.478，2.372-4.011 mg/g，respectively. CONCLUSIONS ： Established method is simple ，accurate and reproducible ，and could be used for the simultaneous determination of 5 components in Huaihua san.
