Time Sequence of Airway Remodeling in a Mouse Model of Chronic Asthma: the Relation with Airway Hyperresponsiveness.
10.3346/jkms.2007.22.2.183
- Author:
Seung Joon KIM
1
;
Chi Hong KIM
;
Joong Hyun AHN
;
Myung Sook KIM
;
Seok Chan KIM
;
Sook Young LEE
;
Soon Seog KWON
;
Young Kyoon KIM
;
Kwan Hyoung KIM
;
Hwa Sik MOON
;
Jeong Sup SONG
;
Sung Hak PARK
Author Information
1. Division of Pulmonology, Department of Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul, Korea. cmcpsh@catholic.ac.kr
- Publication Type:Original Article
- Keywords:
Asthma;
Disease Models, Animal;
Mice;
Bronchial Hyperreactivity;
Chronic Disease
- MeSH:
Time Factors;
Pneumonia/chemically induced/*immunology;
Ovalbumin;
Mice, Inbred BALB C;
Mice;
Lung/drug effects/*immunology;
Female;
*Disease Models, Animal;
Cytokines/*immunology;
Chronic Disease;
Asthma/chemically induced/*immunology;
Animals
- From:Journal of Korean Medical Science
2007;22(2):183-191
- CountryRepublic of Korea
- Language:English
-
Abstract:
During the course of establishing an animal model of chronic asthma, we tried to elucidate the time sequence of airway hyperresponsiveness (AHR), airway inflammation, airway remodeling, and associated cytokines. Seven-week-old female BALB/c mice were studied as a chronic asthma model using ovalbumin (OVA). After sensitization, mice were exposed twice weekly to aerosolized OVA, and were divided into three groups depending on the duration of 4 weeks, 8 weeks, and 12 weeks. At each time point, airway responsiveness, inflammatory cells, cytokines in bronchoalveolar lavage fluids (BALF), serum OVA-specific IgE, IgG1, IgG2a, and histological examination were carried out. AHR to methacholine, increased levels of OVA-specific IgG1 and IgG2a, and goblet cell hyperplasia were continuously sustained at each time point of weeks. In contrast, we observed a time-dependent decrease in serum OVA-specific IgE, BALF eosinophils, BALF cytokines such as IL-13, transforming growth factor-beta1, and a time-dependent increase in BALF promatrix metalloproteinase-9 and peribronchial fibrosis. In this OVA-induced chronic asthma model, we observed airway remodelings as well as various cytokines and inflammatory cells being involved in different time-dependent manners. However, increased airway fibrosis did not directly correlate with a further increase in airway hyperresponsiveness.
