Detection of enteroviruses during a 2018 hand, foot and mouth disease outbreak in Malaysia
https://doi.org/10.47665/tb.38.1.026
- Author:
Lee, M.H.P.
1
;
Chong, Y.M.
1
;
Tay, C.G.
2
;
Koh, M.T.
2
;
Chem, Y.K.
3
;
Noordin, N.
3
;
Jahis, R.
4
;
Sam, I.C.
1
;
Chan, Y.F.
1
Author Information
1. Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia
2. Department of Paediatrics, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia
3. National Public Health Laboratory, Sungai Buloh, Selangor, Malaysia
4. Zoonosis Sector, Disease Control Division, Ministry of Health, Putrajaya, Malaysia
- Publication Type:Journal Article
- Keywords:
hand, foot and mouth disease;
HFMD;
enterovirus;
coxsackievirus A6;
Malaysia.
- From:Tropical Biomedicine
2021;38(No.1):150-153
- CountryMalaysia
- Language:English
-
Abstract:
Hand, foot, and mouth disease (HFMD) is a common childhood disease caused by
enteroviruses. In 2018, a HFMD outbreak in Malaysia affected over 76,000 children. In this
study, we used RT-qPCR and CODEHOP PCR to detect the causative agents in 89 clinically
diagnosed HFMD patients in Kuala Lumpur and Selangor. Most (62.9%) of the children were
below 3 years old. PCR with either assay detected enteroviruses in 84.2% (75/89) and CODEHOP
PCR successfully typed 66.7% (50/75) of the enteroviruses. Sequencing of CODEHOP amplicons
showed co-circulation of multiple enteroviruses with coxsackievirus A6 (CV-A6) and A16 as
the predominant serotypes, but not the neurovirulent enterovirus A71. CV-A6 infection was
more common in children less than 12 months old (p=0.01) and was more likely to cause
vesicles in the gluteal area (p=0.01) compared to other enteroviruses. Establishing a robust
identification method during HFMD outbreaks is important for patient management and
public health responses.
- Full text:8.2021my1224.pdf
