Ovicidal Efficacy of Metarhizium anisopliae (Hypocreales: Clavicipitaceae) towards Rhipicephalus sanguineus (Acari: Ixodidae) Eggs
https://doi.org/10.47665/tb.38.1.017
- Author:
Nur Izzati, Z.A.
1
;
Syazwan, S.A.
2
,
3
;
Lee, S.H.
4
;
Ruhil Hayati, H.
1
;
Peng, T.L.
1
Author Information
1. Faculty of Veterinary Medicine, Universiti Malaysia Kelantan, Locked bag 36, Pengkalan Chepa, 16100 Kota Bharu, Kelantan, Malaysia
2. Mycology and Pathology Laboratory, Forest Health and Conservation Programme, Forest Research Institute Malaysia, 52109 Kepong, Selangor, Malaysia&
3. Forest Biotechnology Laboratory, Department of Forest Management, Faculty of Forestry, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
4. Institute of Tropical Forestry and Forest Products, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
- Publication Type:Journal Article
- Keywords:
Entomopathogenic Fungus;
LC50;
Native strain;
Tick eggs;
Biological control.
- From:Tropical Biomedicine
2021;38(No.1):102-105
- CountryMalaysia
- Language:English
-
Abstract:
Rhipicephalus sanguineus, commonly known as brown dog tick is a widespread species with
considerable public health and economic importance. Tremendous efforts were performed
to control the tick populations with the concern of resistance build-up and environmental
issues. Alternative towards microbial control thus emerged as one option to reduce tick
populations. In this study, the ovicidal efficacy of a native isolate entomopathogenic
hyphomycetes fungi, Metarhizium anisopliae strain HSAH5 was evaluated against eggs of
R. sanguineus. Spray applications with three different conidial concentrations of 105, 106 and
107 conidia mL-1; 40 ppm of Flumethrin and a negative control. The M. anisopliae strain was
found highly virulent to R. sanguineus eggs by reducing the hatching percentages to ≈30%
compared with 8.9% in Flumethrin eggs. The result showed a significantly higher mortality in
M. anisopliae group than those of the control groups (F = 42.08, df = 32, P < 0.001) at 30 days
post-infection. However, there are no significant differences within the M. anisopliae group,
in which the mortality between different conidial concentrations is almost the same. The
estimated LC50 of M. anisopliae against eggs of R. sanguineus is 1.36 × 103 conidia ml-1. Thus,
these results suggest M. anisopliae strain HSAH5 could be a potential biocontrol agent of
R. sanguineus in the integrated approach to managing ticks in the residential landscape by
targeting on the eggs.
- Full text:8.2021my1216.pdf
