Acceleration of cutaneous wound healing by Lucilia sericata maggots in diabetic Wistar rats
https://doi.org/10.47665/tb.38.1.015
- Author:
Borkataki, S.
1
;
Katoch, R.
1
;
Goswami, P.
2
;
Bhat, A.
3
;
Chakraborty, D.
4
Author Information
1. Division of Veterinary Parasitology, Faculty of Veterinary Sciences, SKUAST-Jammu, R S Pura, Jammu, India
2. Division of Veterinary Pathology, Faculty of Veterinary Sciences, SKUAST-Kashmir, Shuhama, Srinagar, India
3. Division of Veterinary Microbiology, Faculty of Veterinary Sciences, SKUAST-Kashmir, Shuhama, Srinagar, India
4. Division of Animal Genetics and Breeding, Faculty of Veterinary Sciences, SKUAST-Jammu, R S Pura, Jammu, India
- Publication Type:Journal Article
- Keywords:
Cutaneous wound;
diabetes, healing;
infected;
Luciliasericata;
maggot;
rat.
- From:Tropical Biomedicine
2021;38(No.1):86-93
- CountryMalaysia
- Language:English
-
Abstract:
The study was aimed to evaluate the effectiveness of maggot therapy in healing of cutaneous
infected wound in streptozotocin (STZ) induced diabetic Wistar rat. For live maggots, the
sterilized eggs of Lucilia sericata were obtained from colonies established in laboratory.
Diabetes model was established in 48 male Wister rat by intra-peritoneal injection of STZ
at the dose of 60 mg/kg body-weight. Cutaneous wounds exposed with mixed colonies of
bacteria like Staphylococcus aureus, E. coli and Pseudomonas aeruginosa were prepared in all rat.
The animals equally divided in 4 groups with 12 rats each being presented as treatment
group of control, antibiotic, maggot and maggot with antibiotic in combination. All treatments
were done once and hold for 24 hours. Wound kinetics and bacterial bio burden were
measured at weekly interval to till complete healing. Significant reduction in wound area
with maximum contraction was found (>95%) in maggot treated group when compared to
antibiotic treated (79%) and control (72%). In maggot as well as maggot and antibiotic in
combination group showed early elimination of bacterial bio-burden 7.88±0.03log CFU/ml
to 1.12±0.65log CFU/ml and 7.86±0.04) log CFU/ml to 1.54±0.52log CFU/ml respectively in
three weeks of time. Early healing indication was also experienced on histomorphological
examination of wounded tissue of maggot treated groups by early and better
epithelialization, collagenation and neovascularization with complete healing of wound in
three weeks in comparison to antibiotic and control respectively. However, the present study
did not show any difference in healing of wound with use of maggot alone or in antibiotic
combination. Live maggot of Lucilia sericata effectively lower bacterial bioburden and and
accelerate healing of infected cutaneous wound in diabetic conditions.
- Full text:8.2021my1214.pdf
