In vitro Evaluation of Anti-Chikungunya Virus Activities of Tualang Honey
https://doi.org/10.47665/tb.38.1.008
- Author:
Barkhadle, N.I.
1
;
Mohamud, R.
2
;
Mat Jusoh, T.N.A.
1
;
Shueb, R.H.
1
,
3
Author Information
1. Department of Medical Microbiology and Parasitology, School of Medical Sciences, Health Campus, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia&
2. Department of Immunology, School of Medical Sciences, Health Campus, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia
3. Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia
- Publication Type:Journal Article
- Keywords:
Chikungunya, Tualang honey, antiviral, virucidal.
- From:Tropical Biomedicine
2021;38(No.1):42-49
- CountryMalaysia
- Language:English
-
Abstract:
Chikungunya is an infection caused by chikungunya virus (CHIKV). Although chikungunya has
affected many countries in recent times, specific treatment or licensed vaccine are nonexistent. In this study the potential antiviral properties of Tualang honey against in vitro
CHIKV infection was evaluated. Cytotoxic test was performed using the XTT Cell Viability
assay to determine maximum non-toxic dose (MNTD) in Vero cells. Using plaque assay, the
potential antiviral activities of Tualang honey at various non-toxic concentrations and
treatment regimens were evaluated. Tualang honey demonstrated virucidal effect with
maximum inhibition CHIKV observed was 99.71% (p< 0.05). Tualang honey also had a
prophylactic property by conferring protection to Vero cells during pre-treatment assay,
resulting in up to 98.22% reduction of CHIKV replication under certain treatment regimen.
Furthermore, Tualang honey exhibited anti-viral activities, with as much as 94.87% inhibition
following post-treatment assay of Tualang honey in CHIKV-infected Vero cells. Additionally,
Tualang honey also affected viral entry up to 82.21% after 48 hours of infection. These results
suggest that Tualang honey has wide anti-CHIKV activities in Vero cells and exerts its effect
through different mechanisms although these need to be further validated in other cells or
model of CHIKV infection.
- Full text:8.2021my1208.pdf
