Establishment of a real-time fluorescent quantitative RT-PCR rapid detection method for human astrovirus
10.3760/cma.j.cn112309-20210106-00005
- VernacularTitle:人星状病毒实时荧光定量RT-PCR快速检测方法的建立
- Author:
Yue DU
;
Sai TIAN
;
Yinxia LI
;
Hongbo LIU
;
Shaofu QIU
;
Guangcai DUAN
- From:
Chinese Journal of Microbiology and Immunology
2021;41(3):195-200
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a rapid detection method for human astrovirus based on TaqMan-probe real-time fluorescent quantitative reverse transcription-polymerase chain reaction (RT-PCR).Methods:According to the conservative sequence of human astrovirus ORF1 b gene, we designed the amplification primers and specific fluorescent probe to establish the human astrovirus TaqMan real-time fluorescent quantitative RT-PCR rapid detection method. The specificity, sensitivity and stability of the method were evaluated. We also used this method to detect human astrovirus in clinical samples. Results:The established human astrovirus TaqMan real-time fluorescent quantitative RT-PCR detection method has good specificity and repeatability for human astrovirus, and the sensitivity can reach 10 2 copies/μl. After testing the clinical samples, the detection rate of human astrovirus by our method was 100%. Conclusions:The human astrovirus TaqMan real-time fluorescent quantitative RT-PCR detection method established in this study is simple, rapid, sensitive, specific and stable. It can be used for clinical human astrovirus detection and epidemiological investigation.
