Expression of Survivin and HIAP-1 in Korean Gastric Cancers.
10.5230/jkgca.2003.3.1.19
- Author:
Chan Jin PARK
1
;
Seung Wan RYU
;
In Hoo KIM
;
Won Ki BAEK
;
Seong Il SUH
;
Min Ho SUH
;
Soo Sang SOHN
Author Information
1. Department of Surgery, Keimyung University School of Medicine, Daegu, Korea. sohnss@dsmc.or.kr
- Publication Type:Original Article
- Keywords:
Gastric cancer;
Survivin;
HIAP-1
- MeSH:
Antibodies;
Apoptosis;
Blotting, Western;
Carcinogenesis;
Cell Cycle;
Cell Line;
Cell Survival;
Immunohistochemistry;
Reverse Transcription;
Stomach Neoplasms*
- From:Journal of the Korean Gastric Cancer Association
2003;3(1):19-25
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Dysregulation of apoptosis may attribute to development of cancer by abnormally prolonging cell viability with accumulation of transforming mutations. Survivin and HIAP (Human Inhibitors of Apoptosis)-1 were recently described as apoptosis inhibitors. Their pathogenic roles in gastric cancer are largely unknown. In the present study, we examined the expression of survivin and HIAP-1 in gastric cancer tissues and cell lines in order to elucidate the roles of survivin and HIAP-1 in the process of gastric carcinogenesis. MATENRIALS AND METHODS: Eight gastric cancer cell lines and five gastric cancer tissues were studied. The expression of survivin and HIAP-1 were evaluated by reverse transcription -polymerase chain reaction (RT-PCR), immunohistochemistry, and Western blot. RESULTS: Western blot and RT-PCR analysis revealed survivin and HIAP-1 expression in all gastric cancer cell lines. Increased expression of survivin and HIAP-1 were found in all cases of gastric cancer tissues compared to normal tissues by Western blot analysis. In immunohistochemical analysis tumor cells were stained with anti-survivin and anti-HIAP-1 antibodies. Cell cycle dependence of survivin expression was preserved in gastric cancer cell lines. CONCLUSION: The results indicate that increased expression of survivin and HIAP-1 genes may play an important role in gastric cancer.
