Modified technique of ureter implantation for urinary tract reconstruction in murine renal transplantation model
10.3760/cma.j.cn421203-20200710-00224
- VernacularTitle:改良输尿管置入膀胱尿路重建法建立小鼠肾移植模型
- Author:
Yi YU
;
Zirong BI
;
Dong WANG
;
Jun LI
;
Qifeng JIANG
;
Shicong YANG
;
Qian FU
;
Longshan LIU
;
Changxi WANG
- From:
Chinese Journal of Organ Transplantation
2021;42(1):48-51
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the application value of modified technique of ureter implantation in murine renal transplantation.Methods:Thirty left donor kidneys from BALB/c mice was transplanted into syngeneic mice. Cuff technique was applied for anastomosing kidney artery and vein. The procedure of ureter-bladder anastomoses shifted from implication-fixation-embedding to fixation-implication-embedding. Operative duration, recipient survival rate and complications were recorded.Results:Time for separating vessels, perfusion and excision of donor graft was (25±3) min, (10±6) s for warm ischemia and (25±5) min for cold ischemia. Time for separating recipient vessels was (12±5) min, (7±1) min for arterial anastomosis, (7±1) min for venous anastomosis, (13±2) min for ureter-bladder anastomosis, (5±1) min for right kidney excision and (5±1) min for abdominal closure. Operative duration was(77±3)min. Twenty-six recipients survived over 3 months. The successful operative rate was 86.7%.Conclusions:With a shorter learning curve, modified technique of ureter implantation is easier and faster so as to reduce the postoperative incidence of urinary tract complications during murine renal transplantation.
