Effects of Cross-Linking Agents on the Stability of Human Acellular Dermal Matrix.
- Author:
Nak Heon KANG
1
;
Young Mook YUN
;
Jong Seol WOO
;
Jae Hyung AHN
;
Jin Young KIM
Author Information
1. Department of Plastic & Reconstructive Surgery, College of Medicine, Chungnam National University, Korea. bell-snow@hanmail.net
- Publication Type:Original Article
- Keywords:
Cross-linking;
Acellular dermal matrix
- MeSH:
Abdomen;
Absorption;
Acellular Dermis;
Animals;
Butylene Glycols;
Collagen;
Dermis;
Ether, Ethyl;
Fibroblasts;
Glutaral;
Humans;
Hydrogen-Ion Concentration;
Imidoesters;
Rats
- From:Journal of the Korean Society of Plastic and Reconstructive Surgeons
2008;35(3):248-254
- CountryRepublic of Korea
-
Abstract:
PURPOSE: Human acellular dermal matrix(ADM) is widely used in the treatment of congenital anomalies and soft tissue deficiencies. But it is rapidly degraded in the body and does not provide satisfactory results. There is a need to improve collagen fiber stability through various methods and ultimately regulate the speed of degradation. METHODS: The ADMs were added with various cross- linking agents called glutaraldehyde, dimethyl 3,3'-dithiobispropionimidate to produce cross-linked acellular dermal matrices. 1,4-butanediol diglycidyl ether solution was applied with a pH of 4.5 and 9.0, respectively. The stability of cross-linked dermal matrix was observed by measuring the shrinkage temperature and the degradation rates. The cross- and non-cross linked dermis were placed in the rat abdomen and obtained after 8, 12 and 16 weeks. RESULTS: The shrinkage temperature significantly increased and the degradation rate significantly decreased, compared to the control(p<0.05). All of cross- linked dermises were observed grossly in 16 weeks, but most of non-cross linked dermis were absorbed in 8 weeks. Histologically, the control group ADM was found to have been infiltrated with fibroblasts and most of dermal stroma were transformed into the host collagen fibers. However, infiltration of fibroblasts in the experiment was insignificant and the original collagen structure was intact. CONCLUSION: Collagen cross-linking increases the structural stability and decreases degradation of acellular dermis. Therefore, decrease in body absorption and increase in duration can be expected.
