Analysis of driver gene mutations in colorectal cancer by using next-generation sequencing

VernacularTitle:二代测序技术检测结直肠癌驱动基因突变分析
Author: Yingying HUANG ; Wenzhuo JIA ; Gang ZHAO ; Xueqing HU ; Ning ZHOU ; Shuai ZHANG ; Yunbo ZHAO ; Lin LI ; Hong SHI
From: Chinese Journal of Geriatrics 2021;40(5):646-649
CountryChina
Language:Chinese
Abstract: Objective:To investigate the characteristics of gene mutations in colorectal cancer(CRC)patients by using next-generation generation sequencing(NGS).Methods:Blood and tissue samples were collected from 90 CRC patients admitted to Beijing Hospital between August 5, 2016 and December 29, 2020.Analysis of driver gene mutations was performed by using a 1021-gene NGS panel.Results:There were 43 tissue samples and 83 blood samples.Also, 36 patients had both tissue and blood samples.The frequency rates of KRAS and BRAF mutations were 51.2%(22/43)and 20.9%(9/43)in tissue samples, and 3 rare concomitant KRAS/ BRAF mutations were detected.The frequency rates of KRAS and BRAF mutations were 26.5%(22/83)and 10.8%(9/83)in blood samples.In patients with tissue and blood samples, the rates of KRAS and BRAF mutations were 52.8%(19/36)and 10.8%(8/36). Conclusions:The rate of KRAS mutations in tissue samples from colorectal cancer patients is similar to rates reported in the literature, but the rate of BRAF mutation and the rate of rare KRAS and BRAF co-mutations are higher than those reported from other countries.