Screening of radiosensitive lipid metabolites in rat small intestine after total body irradiation with 60Co γ-rays
10.3760/cma.j.issn.0254-5098.2021.03.003
- VernacularTitle:60Co γ射线全身照射大鼠的小肠组织中辐射敏感脂质代谢物的筛选
- Author:
Cong XI
;
Hua ZHAO
;
Xue LU
;
Tianjing CAI
;
Mei TIAN
;
Qingjie LIU
- From:
Chinese Journal of Radiological Medicine and Protection
2021;41(3):172-177
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To screen radiosensitive lipid metabolites in rat small intestine and analyze their metabolic pathways, in order to provide scientific basis for radiation enteropathy biomarkers.Methods:The total body irradiation of 60Co γ rays was performed to rats with different doses of 0, 1, 2, 3, 5 and 8 Gy. The changes of lipids in small intestine were studied by targeted lipidomics method based on liquid chromatography coupled mass spectrometry (LC-MS). Results:Fifteen lipids in small intestine were screened as radiosensitive metabolites at 3 d after irradiation, including 4 up-regulated lipids and 11 down-regulated lipids( t=-6.395, 5.998, 5.836, -5.503, -5.449, -5.422, 4.841, 4.802, 4.621, 4.457, 4.426, 4.373, 4.110, 3.945, 3.902, P< 0.05 and FDR < 0.05). The metabolic pathways of sphingolipid, glycerophosphoplipid were significantly enriched. Four phosphatidyl serines (PS)increased while 1 phosphatidic acid(PA), 2 sphingomyelins(SM) and 4 fatty acids(FA)decreased in a good dose-response manner( R2> 0.80, P< 0.05), which were more potential radiation enteropathy biomarkers. Conclusions:Lipid metabolites in rat small intestine were significantly changed after the rat was total body irradiated with 60Co γ-rays.Eleven lipids with good dose-response relationship were more potential to be radiation enteropathy biomarkers.
