Effect of Mitomycin C and 5-Fluorouracil on Cultured Human Nasal Mucosa Fibroblasts.
10.3341/jkos.2011.52.2.233
- Author:
Keun Hae KIM
1
;
In Taek KIM
Author Information
1. Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Korea. kimkh@cu.ac.kr
- Publication Type:In Vitro ; Original Article
- Keywords:
5-fluorouracil;
Mitomycin C;
Nasal mucosa fibroblast
- MeSH:
Antimetabolites;
Apoptosis;
Cell Survival;
Dacryocystorhinostomy;
Fibroblasts;
Fluorouracil;
Humans;
Microscopy, Phase-Contrast;
Mitomycin;
Nasal Mucosa
- From:Journal of the Korean Ophthalmological Society
2011;52(2):233-240
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To investigate the effects of two antimetabolites, mitomycin C (MMC) and 5-fluorouracil (5-FU), on proliferation of cultured human nasal mucosa fibroblasts. METHODS: Human nasal mucosa fibroblasts were primarily cultured, and exposed to various concentrations of MMC and 5-FU for 5 minutes. Control fibroblasts were exposed to only DMEM media without the drugs. Effect of drugs on cell morphology was observed by phase-contrast microscopy. Cell viability and apoptosis were measured using MTT [3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyl-tetrazolium bromide] assay and Acridine orange/Hoechst (AO/HO) staining, respectively. RESULTS: In both experimental groups exposed to MMC and 5-FU, fibroblasts maintained standard spindle shape. The MTT assay showed that both MMC and 5-FU inhibited fibroblast proliferation in a dose dependent manner. AO/HO staining showed apoptotic cells in both experimental groups. CONCLUSIONS: Both MMC and 5-FU have an antiproliferative effect on fibroblasts in vitro at least through induction of apoptosis. Therefore, adjuvant use of either MMC or 5-FU during endonasal dacryocystorhinostomy may improve the clinical outcome by inhibiting proliferation of the nasal mucosa.
