Protective Effects of Immunization with Proteinase, Phospholipase and Cell Wall Proteins of Candida albicans in Mice Candidosis.
- Author:
Woon Seob SHIN
1
;
Sun Ju CHOI
;
Young Chul YANG
;
Su Jung PARK
;
Joo Young PARK
;
Soo Ki KIM
;
Kyoung Ho LEE
Author Information
1. Department of Microbiology, Kwandong University College of Medicine, Kangnung, Korea.
- Publication Type:Original Article
- Keywords:
Candida albicans;
Proteinase;
Phospholipase;
Immunization
- MeSH:
Acrylic Resins;
Animals;
Antibodies;
Candida;
Candida albicans;
Cell Wall;
Chromatography;
Chromatography, High Pressure Liquid;
Chromatography, Liquid;
Chromatography, Reverse-Phase;
Dithiothreitol;
Endopeptidase K;
Ethanolamines;
Fungi;
Glucan Endo-1,3-beta-D-Glucosidase;
Immunization;
Immunization, Passive;
Mice;
Multienzyme Complexes;
Peptide Hydrolases;
Phospholipases;
Proteins;
Survival Rate
- From:Korean Journal of Medical Mycology
2009;14(1):9-15
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: The opportunistic fungus Candida albicans is a major pathogen especially to immunocompromised patients. OBJECTIVES: We examined the protective effect of the active and passive immunizations to evaluate the applicability for the treatment of candidosis in Candida-infected mice model. METHODS: Candida cell wall components were obtained by treatment of lyticase, proteinase K, and dithiothreitol. The proteinase was purified from the culture filtrates of C. albicans using a series of chromatographic steps consisting of DEAE-Sepharose FF, Sephacryl S-200 HR and size-exclusion high performance liquid chromatography. The phospholipase was purified from the culture supernatant of C. albicans with DEAE column chromatography, reverse phase column chromatography, revere phase HPLC and size-exclusion HPLC. Antibodies to cell wall protein components, proteinase and phospholipase were produced by immunization into mice of same strain. RESULTS: The mean survival times of active and passive immunized mice groups were longer than those of non-immunized groups. CONCLUSION: These results showed that immunization with proteinase and its antibody were the most effective to prolong survival time in Candida-infected mice.
