The effective transfection of a low dose of negatively charged drug-loaded DNA-nanocarriers into cancer cells via scavenger receptors
- Author:
Mirza Muhammad Faran Ashraf Baig
1
,
2
,
3
;
Chengfei ZHANG
;
Furqan-Muhammad AKHTAR
;
Ammara SALEEM
;
Jahanzeb MUDASSIR
Author Information
1. Laboratory of Biomedical&Pharmaceutical Engineering of Stem Cells Research,Restorative Dental Sciences,Faculty of Dentistry,The University of Hong Kong,999077,Hong Kong,PR China
2. State Key Laboratory of Analytical Chemistry for Life Sciences,School of Chemistry and Chemical Engineering,Nanjing University,Nanjing,210023,PR China
3. Faculty of Pharmacy,Bahauddin Zakariya University,Multan,60000,Pakistan
- Keywords:
Cisplatin(CPT);
DNA-nanowires(DNA-NWs);
HepG2 resistant cancer cells;
Scavenger receptors
- From:
Journal of Pharmaceutical Analysis
2021;11(2):174-182
- CountryChina
- Language:Chinese
-
Abstract:
DNA-nanotechnology-based nano-architecture scaffolds based on circular strands were designed in the form of DNA-nanowires(DNA-NWs)as a polymer of DNA-triangles.Circularizing a scaffold strand(84-NT)was the critical step followed by annealing with various staple strands to make stiff DNA-triangles.Atomic force microcopy(AFM),native polyacrylamide gel electrophoresis(PAGE),UV-analysis,MTT-assay,flow cytometry,and confocal imaging were performed to assess the formulated DNA-NWs and cisplatin(CPT)loading.The AFM and confocal microscopy images revealed a uniform shape and size distribution of the DNA-NWs,with lengths ranging from 2 to 4 μm and diameters ranging from 150 to 300 nm.One sharp band at the top of the lane(500 bp level)with the loss of electrophoretic mobility during the PAGE(native)gel analysis revealed the successful fabrication of DNA-NWs.The loading efficiency of CPT ranged from 66.85％to 97.35％.MTT and flow cytometry results showed biocompatibility of the blank DNA-NWs even at 95％concentration compared with the CPT-loaded DNA-NWs.The CPT-loaded DNA-NWs exhibited enhanced apoptosis(22％)compared to the apoptosis(7％)induced by the blank DNA-NWs.The release of CPT from the DNA-NWs was sustained at＜75％for 6 h in the presence of serum,demonstrating suitability for systemic applications.The IC50 of CPT@DNA-NWs was reduced to 12.8 nM CPT,as compared with the free CPT solution exhibiting an IC50 of 51.2 nM.Confocal imaging revealed the targetability,surface binding,and slow internalization of the DNA-NWs in the scavenger-receptor-rich cancer cell line(HepG2)compared with the control cell line.
