Single-run reversed-phase HPLC method for determining sertraline content, enantiomeric purity, and related substances in drug substance and finished product
- Author:
Rosetti ALESSIA
1
,
2
;
Ferretti ROSELLA
;
Zanitti LEO
;
Casulli ADRIANO
;
Villani CLAUDIO
;
Cirilli ROBERTO
Author Information
1. National Centre for the Control and Evaluation of Medicines,Chemical Medicines Unit,Istituto Superiore di Sanit(a) (ISS),Viale Regina Elena 299,00161,Rome,Italy
2. Department of Chemistry and Technology of Drugs,Sapienza University of Rome,00185,Rome,Italy
- Keywords:
Chiralpak IG-3;
Enantiomers;
Diastereomers;
Reversed-phase enantioselective HPLC;
Sertraline HCl
- From:
Journal of Pharmaceutical Analysis
2020;10(6):610-616
- CountryChina
- Language:Chinese
-
Abstract:
A direct enantio-, diastereo-, and chemo-selective high-performance liquid chromatographic method was developed for determining the content, enantiomeric purity, and related substances of the chiral antidepressant drug sertraline HCl in a single chromatographic run. The separation was achieved on a chiral stationary phase based on amylose tris(3-chloro-5-methylphenylcarbamate) under reversed-phase conditions. The method was optimized by evaluating the influence of the temperature and mobile phase composition on the retention and selectivity. The application of the single-run approach allowed to baseline resolve all investigated species in less than 15 min, without using buffers or tandem-coupled columns. The chromatographic method was validated according to the guidelines of the Official Medi-cines Control Laboratory and applied to control the content of sertraline HCl and related chiral sub-stances in a generic antidepressant formulation.
