Expression of serum microRNA-195 in patients with esophageal cancer and its clinical significance
10.3760/cma.j.cn115455-20200527-00669
- VernacularTitle:食管癌患者血清微小RNA-195表达及临床意义
- Author:
Dongxia SUI
- From:
Chinese Journal of Postgraduates of Medicine
2021;44(1):58-62
- CountryChina
- Language:Chinese
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Abstract:
Objective:To investigate the expression of serum microRNA (miR)-195 in patients with esophageal cancer and its clinical significance.Methods:The clinical data of 105 patients with esophageal cancer in Beijing Huairou Hospital from April 2015 to April 2019 were retrospective analyzed. Ninety healthy examinees during the same period were selected as the healthy control. The relative expression level of serum miR-195 was detected by real-time quantitative fluorescence polymerase chain reaction. The receiver operating characteristic (ROC) curve was drawn, and the predictive value of serum miR-195 for esophageal cancer was analysis. Kaplan-Meier method was used for survival analysis, and log-rank test was used for comparison. Multivariate Cox analysis was used to analyze the independent risk factors of esophageal cancer prognosis.Results:The relative expression level of serum miR-195 in patients with esophageal cancer was significantly lower than that in healthy control (4.39 ± 1.86 vs. 7.17 ± 2.37), and there was statistical difference ( t = 9.155, P<0.05). The median level of serum miR-195 in patients with esophageal cancer was served as cut-off value.>4.39 was regarded as high expression (50 cases), and ≤ 4.39 was as low expression (55 cases). The relative expression level of serum miR-195 was related to tumor diameter, TNM stage, degree of invasion and lymph node metastasis ( P<0.05); however, there was no correlation with gender, age, pathological type and differentiation degree ( P>0.05). ROC curve analysis result showed that area under curve of serum miR-195 relative expression level in predicting esophageal cancer was 0.819 (95% CI 0.758 to 0.879, Z = 10.265, P<0.05), Yoden index was 0.539, and the optimal cut-off was 6.03, with a sensitivity of 82.86% and specificity of 71.11%. The median survival time in serum miR-195 low expression patients was significantly shorter than that in serum miR-195 high expression patients (25.7 months vs. 36.6 months), 5-year overall survival rate was significantly lower than that in serum miR-195 high expression patients (18.2% vs. 38.0%), and there were statistical differences ( P<0.05). Multivariate Cox analysis result showed that miR-195 and tumor diameter were independent risk factors for the prognosis of patients with esophageal cancer ( HR = 0.377 and 0.418, 95% CI 1.276 to 5.599 and 1.478 to 7.608, P<0.01). Conclusions:The relative expression of serum miR-195 in patients with esophageal cancer is significantly lower than that in healthy people. The lower the expression of miR-195, the worse the prognosis. The miR-195 can be used as one of the indicators for early prediction and prognostic evaluation of esophageal cancer.
