Expression Change of Complement Regulator Genes of the Human Astrocytoma Cell Line after A beta1-42 and Interferon Gamma Administration.
- Author:
Young Sook CHOI
1
;
Sang Ho KIM
Author Information
1. Department of Pathology, College of Medicine, The Catholic University of Korea, Seoul, Korea. complt@catholic.ac.kr
- Publication Type:Original Article
- Keywords:
Alzheimer's Disease;
beta-amyloid protein;
C1 Inhibitor;
Complement Factor I;
Interferon-gamma
- MeSH:
Alzheimer Disease;
Aminopeptidases;
Amyloid beta-Peptides;
Astrocytoma;
Cell Line;
Clusterin;
Complement Factor I;
Complement System Proteins;
Genes, Regulator;
Humans;
Indicators and Reagents;
Interferon-gamma;
Interferons;
Membranes;
RNA, Messenger;
Vitronectin
- From:Journal of the Korean Geriatrics Society
2008;12(4):235-245
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: We determined the changes of complement regulator gene expression in the amyloid-beta1-42(A beta1-42) and interferon-gamma(IFN-gamma)-stimulated human astrocytoma cell line. METHODS: The human astrocytoma cell line, U373MG, was stimulated with IFN-gamma(62.5-1,000U/ml) in the presence or absence of aggregated A beta1-42(1-20micrometer) for 24 hours. Messenger RNA expression of C1 inhibitor(C1-INH), complement factor I(CFI), clusterin, vitronectin, decay accelerating factor(DAF), membrane cofactor protein(MCP), and CD59 was measured by quantitative real-time reverse transcriptase-PCR. RESULTS: IFN-gamma(final concentration, 500U/ml) markedly increased the expression of mRNA for C1-INH in a time dependent fashion. A beta1-42(final concentration, 2micrometer) induced a slight increase in the expression of C1-INH. Messenger RNAs for CFI and clusterin were minimally increased, but other regulators were unchanged or decreased by either A beta1-42 or IFN-gamma. IFN-gamma overrode A beta1-42-induced mRNA expression of C1-INH when the cells were treated with these two reagents together. CONCLUSION: Among the complement regulator genes in the human astrocytoma cell line, U373MG, only C1-INH was significantly up-regulated by IFN-gamma with or without A beta1-42 administration.
