Establishment, evaluation, and determination of saliva glucose concentration by ion chromatography

VernacularTitle:离子色谱测定唾液葡萄糖含量方法的建立及评估
Author: Chun XU ; Qian DOU ; Shiwen WANG ; Zifeng ZHANG ; Qing DAI
From: Chinese Journal of Endocrine Surgery 2021;15(1):61-65
CountryChina
Language:Chinese
Abstract: Objective:To establish an analytical method for measuring the concentration of glucose in saliva by ion chromatography.Methods:The proteins in saliva were removed by thermal denaturation method, CarboPac PA20 (3×30 mm) was used as a protective column and CarboPac PA20 (3×150 mm) was used as an analytical column for ion chromatography analysis. Gradient elution was carried out with A: ultra-pure water, B: 250 mmol/L NaOH solution and C: 500 mmol/L NaAc solution. Pulsed ampere detector was used for detection.Results:This method had a good linear relationship in the range of 0.04 to 0.12 mg/L, with a linear relation coefficient of 0.9967. The detection limit of glucose was 2 μg/L, the mean value of the relative standard deviation (RSD) of the repeatability measurement was 0.75%, and the average spike recovery was 103.07%.Conclusion:This method is simple, sensitive, accurate and stable, and can be used for the determination of glucose concentration in saliva.