Knockout of MIF gene attenuates severe acute pancreatitis-associated lung injury in mice
10.3760/cma.j.issn.1671-0282.2021.05.008
- VernacularTitle:敲除巨噬细胞移动抑制因子基因可减轻小鼠重症急性胰腺炎相关肺损伤
- Author:
Wanpeng WANG
;
Bo CHENG
;
Shujun YANG
;
Yanna LIU
;
Qiaofang WANG
;
Yaodong SONG
;
Changju ZHU
- From:
Chinese Journal of Emergency Medicine
2021;30(5):551-556
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the role of macrophage migration inhibitory factor (MIF) in severe acute pancreatitis (SAP) associated lung injury in mice.Methods:Totally 32 mice were randomly divided into 4 groups ( n=8/per group): wild type control group (WT+CON group), wild type SAP group (WT+SAP group), MIF gene knockout control group (KO+CON group), and MIF gene knockout SAP group (KO+SAP group). SAP model was established by intraperitoneal injection of L-arginine (4 mg/g). The expression of serum amylase, IL-6, TNF-α and MIF were detected by ELISA. The pathological changes of pancreatic and lung tissues were observed by HE staining. The expression of IL-6 and TNF-α in lung tissue was detected by immunohistochemistry. The expression of NF-κB in lung tissue was detected by Western blot. For measurement data, t test was used for comparison between two groups, and one-way analysis of variance was used for comparison between multiple groups. Results:Compared with the WT+CON group, pathological score of pancreatic and lung injury, serum amylase, TNF-α and IL-6 expression in serum and lung tissues were significantly increased in the WT+SAP group ( P<0.05), while the above indexes were significantly decreased in the KO+SAP group ( P<0.05). In addition, the expression of NF-κB protein in KO+SAP group was significantly lower than that in the WT+SAP group ( P<0.05). Conclusions:MIF gene knockout can alleviate severe acute pancreatitis associated acute lung injury in mice, and its mechanism may be related to NF-κB.
