Effects of psoralen on the proliferation, invasion and migration of HTB-47 and CRL-1932 renal cancer cell lines and related mechanisms
10.3760/cma.j.cn121382-20200911-00106
- VernacularTitle:补骨脂素对HTB-47和CRL-1932肾癌细胞系的增殖、侵袭和迁移的影响及相关机制
- Author:
Lingxing YUAN
;
Xiufeng LI
;
Chuanlan GU
;
Shijun LU
;
Qingyan LOU
;
Yuan LIU
;
Fanlu LIN
;
Yingmin XU
- From:
International Journal of Biomedical Engineering
2021;44(1):34-38
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effect of psoralen on the proliferation, invasion and migration of HTB-47 and CRL-1932 renal cancer cells cultured in vitro, and to further explore the internal mechanism of psoralen inhibiting renal cancer.Methods:The experimental group was HTB-47 and CRL-1932 renal cancer cells treated with dimethyl sulfoxide solution containing 30 μg/ml psoralen, and the control group was renal cancer cells treated with dimethyl sulfoxide. Scratch test, CCK8, Transwell, and Western blot were used to detect the effect of psoralen on renal cancer cells.Results:Compared with the control group, the proliferation, invasion and migration of renal cancer cells treated with psoralen in the experimental group were significantly inhibited. In the renal cancer cells treated with psoralen, the protein expression levels of MKI67, PCNA, MMP2 and MMP9 were significantly decreased (all P<0.05). Conclusions:Psoralen can significantly inhibit the proliferation, invasion and migration of HTB-47 and CRL-1932 renal cancer cells in vitro. The mechanism may be to inhibit the progression of renal cancer by regulating MKI67, PCNA, MMP2 and MMP9.
