Genotoxicity assessment of three reference chemicals in rat hepatocytes by alkaline comet assay
10.19428/j.cnki.sjpm.2021.19610
- VernacularTitle:大鼠体内肝细胞碱性彗星试验评估3种参考化学物的遗传毒性
- Author:
Ning WANG
1
;
Wen-zhong ZHANG
2
;
Yu-li SUN
1
;
Nai-min SHAO
1
;
Xue-qing CHENG
1
;
Jun-li LIU
1
;
Yu DING
1
;
Na-na SUN
2
;
Xin-yu HONG
1
Author Information
1. Shanghai Municipal Center for Disease Control and Prevention， Shanghai 201611， China
2. China National Center for Food Safety Risk Assessment， Beijing 100022， China
- Publication Type:Research Article
- Keywords:
alkaline comet assay of hepatocyte in rats;
ethyl methanesulfonate;
N-methyl-N-nitrosourea;
D-mannitol;
genotoxicity
- From:
Shanghai Journal of Preventive Medicine
2021;33(7):624-629
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a comet test method for detection of genotoxicity of three reference chemicals in rat liver cells. Methods:6-10 week old Sprague Dawley rats were randomly divided into 4 groups， with normal saline （0.9% NaCl solution） as negative control group. Animals in three test groups were treated， respectively， with ethyl methanesulfonate （EMS） 200 mg/kg， N-methyl-N-nitrosourea （MNU） 50 mg/kg， and D-mannitol 2 000 mg/kg. There were 10 animals in each group， 5 males and 5 females. The animals received two times （21 h interval） of test compounds through intragastric administration， and their clinical symptoms and body weight changes were recorded during the experiment. The rats were sacrificed 3 h after the last exposure. The liver was weighed， then used to prepare single-cell suspensions for the alkaline comet test which determines the average tail DNA content percentage （DNA%） of hepatocytes and other comet indicators. Results:（1） D-mannitol， EMS and MNU did not show significant toxicity in the whole animal. （2） The mean values of tail DNA content percentage （DNA%） of rat hepatocytes in EMS ［（60.07±24.69）%］ and MNU ［（41.66±22.35）%］ groups were higher than that in the negative control group ［（2.32±1.39）%］ and the difference was statistically significant （P<0.05）. The difference between D-mannitol group ［（3.06±3.30）%］ and the negative control group was not significant （P>0.05）. Conclusion:This laboratory has established a comet test method using hepatocytes from treated rats. Among three testing chemicals， EMS and MNU have displayed genotoxicity by this assay， but no genotoxicity was observed in D-mannitol treated animals.
