LINC00519 regulates the proliferation, apoptosis, migration and invasion of gastric cancer cells through the miR-876-3p/HMGA1 axis
DOI：10.3872/j.issn.1007-385x.2021.06.004
- VernacularTitle:LINC00519通过miR-876-3p/HMGA1轴调控胃癌细胞的增殖、凋亡、迁移和侵袭
- Author:
ZHANG Like
1
,
2
;
WANG Jianguang
1
,
2
;
JING Dongshuai
1
,
2
;
LEI Liangliang
1
,
2
;
LIU Dechun
1
,
2
Author Information
1. Department of Gastrointestinal Surgery, the First Affiliated Hospital of Henan University of Science and Technology
2. School of Clinical Medicine, Henan University of Science and Technology, Luoyang 471003, Henan, China
- Publication Type:Journal Article
- Keywords:
LINC00519；miR-876-3p；高迁移率蛋白A1；胃癌；增殖；凋亡；迁移；侵袭
- From:
Chinese Journal of Cancer Biotherapy
2021;28(6):574-581
- CountryChina
- Language:Chinese
-
Abstract:
[摘 要] 目的：探讨长基因间非编码RNA 00519（long intergene non-coding RNA 00519，LINC00519）调控miR-876-3p/高迁移率家族蛋白A1（high mobility group protein A1，HMGA1）轴在胃癌HGC-27细胞的增殖、凋亡、迁移和侵袭中的作用。方法：采用qPCR检测胃癌细胞HGC-27和胃黏膜上皮细胞GES-1中LINC00519的表达水平。将HGC-27细胞按转染处理分为si-NC、si-LINC00519、si-LINC00519+anti-miR-NC和si-LINC00519+anti-miR-876-3p组，采用集落形成实验检测细胞克隆形成能力，流式细胞术检测细胞凋亡和周期分布，Transwell实验检测细胞迁移和侵袭。双荧光素酶报告实验和qPCR验证LINC00519与miR-876-3p、miR-876-3p与HMGA1之间的相互作用。结果：HGC-27细胞中LINC00519表达较GES-1细胞显著升高（P<0.05），转染siRNA后si-LINC00519组HGC-27细胞中LINC00519的表达水平较si-NC组显著降低（t=47.294，P<0.01）。与si-NC组比较，si-LINC00519组HGC-27细胞克隆数、迁移侵袭数、S期细胞比例均显著降低（均P<0.01），凋亡率、G0/G1期细胞比例均显著升高（均P<0.01）。与si-LINC00519+anti-miR-NC组比较，si-LINC00519+anti-miR-876-3p组HGC-27细胞克隆数、迁移侵袭数、S期细胞比例升高（均P<0.01），凋亡率、G0/G1期细胞比例显著降低（均P<0.01）。LINC00519能够靶向负调控miR-876-3p的表达，miR-876-3p靶向负调控HMGA1的表达。结论：敲降LINC00519能够通过调控miR-876-3p/HMGA1轴抑制胃癌HGC-27细胞的增殖、迁移和侵袭，诱导细胞凋亡。
- Full text:20210604.pdf
