Optimization of determination method for silver sulfadiazine cream and its stability under extreme conditions
10.12206/j.issn.1006-0111.202012011
- VernacularTitle:磺胺嘧啶银乳膏含量测定方法的优化和极端环境下的稳定性考察
- Author:
Ling LI
1
;
Hongjie SONG
2
;
Deying DING
1
;
Lixin SHU
1
Author Information
1. Naval Medical University: School of Pharmacy, Shanghai 200433, China.
2. Naval Medical University: Department of Pharmacy, Chang Hai Hospital, Shanghai 200438, China.
- Keywords:
silver sulfadiazine;
content determination;
stability;
HPLC
- From:
Journal of Pharmaceutical Practice
2021;39(4):345-347
- CountryChina
- Language:Chinese
-
Abstract:
Objective To optimize the method of assay for silver sulfadiazine, and to investigate its stability in high temperature, humidity and salt environment. Methods Agilent ZORBAX SB-C18 column was used in HPLC for the determination of silver sulfadiazine with acetonitrile and 0.1% phosphoric acid as mobile phase.The flowing rate was 1.0 ml/min and column temperature was 30℃. Salt spray test chamber was used to simulate the high temperature, humidity and salt environment. The silver sulfadiazine content was assayed at 0, 2, 4, 6, 8, 10, 12 weeks. Results The calibration curve was linear within the range of 4.00～20.00 μg/ml (r=0.9999). The average recovery was 101.47% (RSD=2.33%). The content of silver sulfadiazine cream began to decrease significantly after 4 weeks. Conclusion This method is convenient, accurate and reliable. It can be used for the content determination of silver sulfadiazine in the cream.The results showed that the silver sulfadiazine cream was unstable in the environment of high temperature, humidity and salt. Therefore, environmental impact should be fully considered in transportation, storage and application. For the long-distance navigation mission, protective measures should be taken for its packaging or replace it with more stable products.
