The anti-inflammatory role of α7 nicotinic acetylcholine receptor in microglial cells and its mechanisms
10.12206/j.issn.1006-0111.202103028
- VernacularTitle:α7n型乙酰胆碱能受体在小胶质细胞中下调炎症水平的作用及其机制研究
- Author:
Yue SHEN
1
;
Jingjing ZHANG
2
;
Jing DU
3
;
Qi CAO
2
;
Chong LIU
2
;
Jiao QIAN
1
Author Information
1. a. Department of Pharmacy, Changhai Hospital, 1b. School of Pharmacy, Naval Medical University, Shanghai 200433, China.
2. 1b. School of Pharmacy, Naval Medical University, Shanghai 200433, China.
3. School of Pharmacy, Jining Medical University, Jining 276826, China.
- Keywords:
α7 nicotinic acetylcholine receptor;
microglial cells;
inflammation;
autophagy
- From:
Journal of Pharmaceutical Practice
2021;39(4):340-344
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the anti-inflammatory role of α7 nicotinic acetylcholine receptor (α7nAChR) under inflammatory stress and its mechanisms. Methods PNU282987 was used for the activation of α7nAChR and LPS was administrated as inflammatory stressor. Realtime PCR was used for the detection of IL-1β, IL-6, TNF-α, M1 macrophage marker CD68, CD86 and M2 macrophage marker CD206, Arg1. Cell immunofluorescence was used for the detection of M1/M2 ratio and Western blot was applied for the detection of autophagy-related proteins. Results Under the stimulation of LPS, the mRNA levels of proinflammatory cytokines IL-1β, IL-6 and TNF-α, the proportion of M1 macrophage and autophagy process were increased in BV2 microglial cells. However, the administration of PNU282987 significantly decreased the mRNA levels of IL-1β, IL-6 and TNF-α and the proportion of M1 macrophage while increased the proportion of M2 macrophage and the level of autophagy process. Conclusion Activating α7nAChR plays an anti-inflammatory role in microglial cells under inflammatory stress due to the regulation of M1/M2 macrophage ratio and increase of autophagy level.
