Determination of harpagide and harpagoside contents in Scrophularia ningpoensis from 15 origins
10.12206/j.issn.1006-0111.202008102
- VernacularTitle:15个产地玄参中哈巴苷与哈巴俄苷含量测定
- Author:
Dan REN
1
,
2
;
Fangyuan QI
3
;
Ziyan HUANG
3
;
Luping QIN
1
,
2
;
Bo ZHU
3
Author Information
1. School of Pharmacy, Zhejiang Chinese Medical University, Hangzhou 310053, China
2. College of Pharmacy, Shaanxi University of Chinese Medicine, Xianyang 712046, China.
3. School of Pharmacy, Zhejiang Chinese Medical University, Hangzhou 310053, China.
- Keywords:
Scrophularia ningpoensis;
Harpagide;
Harpagoside;
HPLC
- From:
Journal of Pharmaceutical Practice
2021;39(4):313-316
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an HPLC method for the simultaneous determination of harpagide and harpagoside content in Scrophularia ningpoensis (SN). Methods An Eclipse C18 column was used for determination of methanol extract of S. ningpoensis with a HPLC-PDA method and mobile phase of acetonitrile-0.03% phosphate solution in a gradient elution manner. The flow rate of mobile phase was 1.0 ml/min, and the detection wavelengths were 210 nm and 280 nm. Results Harpagide and harpagoside contents in SN showed good linear relationships within 0.1020-0.5100 mg/ml (r=0.9999) and 0.0340-0.1700 mg/ml (r=0.9999). Their average recovery rates were 97.44% and 97.08%.The RSDs were 0.93% and 1.24%.. There were significant differences in the contents of harpagide and harpagoside in SN from 15 origins (P<0.01). The content of harpagoside in Sichuan Long-dong, Zhejiang Lin-an, Zhejiang Pan-an and Henan Nan-feng is higher. Conclusion This method is stable, accurate and reproducible and can be used for the quality control of SN.
