Content determination and extraction process of macrocyclic polyphenols from Penthorum chinense Pursh
10.12206/j.issn.1006-0111.202009048
- VernacularTitle:赶黄草中大环多酚类成分含量测定及其提取工艺
- Author:
Lan CHEN
1
;
Shenggui JIANG
2
;
Pengjie SHI
2
;
Shuxin CAI
2
;
Zhiying DONG
2
;
Lianna SUN
2
Author Information
1. Sanatorium Area 3, Air Force Hangzhou Special Service Recuperation Center, Hangzhou 310002, China.
2. School of Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
- Keywords:
Penthorum chinense Pursh;
HPLC;
PHG;
PGHG;
THA;
orthogonal method;
extraction process
- From:
Journal of Pharmaceutical Practice
2021;39(4):309-312
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an HPLC method for simultaneous assay of macrocyclic polyphenols from Penthorum chinense Pursh, pinocembrin-7-O-[4'', 6''-(S)-hexahydroxydiphenoyl]-β-D-glucose (PHG), pinocembrin-7-O-[3''-O-galloyl-4'', 6''-(S)-hexahydroxydiphenoyl]-β-D-glucose (PGHG) and pinocembrin dihydrochalcone-7-O-[3''-O-galloyl-4'', 6''-(S)-hexahydroxydiphenoyl]-β-D-glucoside or thonningianin A (THA), and optimize the extraction process. Methods The total extraction rate of PHG, PGHG, THA was used as an investigated index to analyze the extracts from Penthorum chinense Pursh. Orthogonal design was applied to evaluate solvent amount, extraction time, solvent concentration and extraction times as the influencing factors for the optimal extraction process of macrocyclic polyphenols from Penthorum chinense Pursh. Results When this content assay method was adopted, there were good linear relationships for PHG, PGHG, THA in the linear range. The recoveries were between 100.90% to 102.04% with the RSDs below 1.5%. The optimal extraction process was involved in cutting Penthorum chinense Pursh into 3-5 cm, adding 10 times 80% ethanol aqueous solution by volume and refluxing 2 hours twice. The extraction rate of macrocyclic polyphenols was above 90% with this process. Conclusion This assay method is accurate, stable, and repeatable. The optimized extraction process is stable and feasible for further development and utilization.
