Simultaneous determination of 14 chlorinated hydrocarbons in urine by headspace gas chromatography-mass spectrometry
10.11763/j.issn.2095-2619.2020.04.019
- Author:
Xiaoxuan CAI
1
;
Si TANG
1
;
Jianmei PENG
1
;
Xiaozhou ZHUANG
1
;
Lihe YE
1
;
Siyan LI
1
;
Yuanqin ZHONG
1
;
Zheng MA
1
;
Huipeng CHEN
1
;
Fenghua ZHANG
1
Author Information
1. Dongguan Prevention and Treatment Center for Occupational Disease, Dongguan City Key Laboratory of Occupational Health Dectection Dongguan,Guangdong 523008,China
- Publication Type:Journal Article
- Keywords:
Urine;
Chlorinated hydrocarbons;
Gas chromatography-mass spectrometry;
Headspace analysis
- From:
China Occupational Medicine
2020;47(04):470-474
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish a headspace gas chromatography-mass spectrometry method for the determination of 14 chlorinated hydrocarbons in urine. METHODS: The urine sample 4.00 mL and anhydrous sodium sulfate 3.00 g were added into a 10.00 mL headspace bottle, then the headspace bottle was maintained at 70 ℃ for 40.0 min. After headspace pretreatment, 14 chlorinated hydrocarbons in headspace air were separated in the DB-5 MS capillary column of the gas chromatography and detected by mass spectrometer. RESULTS: There was a good linear relationship of 14 chlorinated hydrocarbons in urine in the range of 0.62-1 630.00 μg/L. The linear correlation coefficient was greater than 0.999 0.The minimum detectable concentration was 0.19-0.43 μg/L and the minimum quantitative concentration was 0.62-1.44 μg/L. The average recovery rate was 89.8%-107.1%. The within-run relative standard deviation(RSD) was 4.0%-8.5% and the between-run RSD was 6.3%-9.1%. Urine samples can be stored at 4 ℃ or-8 ℃ for 3 days and below-20 ℃ for 7 days. CONCLUSION: This method is rapid, simple, sensitive, accurate and has little interference,which can be used as a method for detecting 14 kinds of chlorinated hydrocarbons in urine samples of patients with occupational poisoning.
