Determining 6 arsenic species in human urine by high performance liquid chromatography-atomic fluorescence spectrometry
10.11763/j.issn.2095-2619.2020.02.016
- Author:
Lezhou ZHOU
1
;
Lu YANG
1
;
Sheng FU
1
Author Information
1. Hunan Prevention and Treatment Institute for Occupational Disease Changsha, Hunan 410007, China
- Publication Type:Journal Article
- Keywords:
High performance liquid chromatography;
Atomic fluorescence spectrometry;
Arsenic;
Urine
- From:
China Occupational Medicine
2020;47(02):204-208
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish a methodology using high performance liquid chromatography-atomic fluorescence spectrometry(HPLC-AFS) for the determination of 6 arsenic species including arsenite, dimethyl arsenic acid, monomethyl arsenic acid, dimethyl arsenic acid, monomethyl arsenic acid and arsenite in human urine. METHODS: The PRP-X100 anion exchange chromatographic column was used with 4.0 mmol/L ammonium carbonate, ammonium sulfate and ammonium phosphate mixed solution with pH 8.9 as mobile phase. The carrier current was hydrochloric acid with volume fraction of 10.0% and reducing agent was potassium borohydride with mass fraction of 3.5%. After centrifugal separation of urine sample, 6 forms of arsenics were detected by HPLC-AFS. RESULTS: The linear range of 6 forms of arsenics in urine was 3.90-100.00 μg/L. The correlation coefficient was 0.999 1-0.999 9, and the detection limit was 1.31-3.90 μg/L. The recovery rate was 91.2%-103.5%. The within-run and between-run relative standard deviations were 0.5%-4.9% and 1.8%-5.0%, respectively. The samples could be stored for at least 7 days under the temperature of-80 ℃. CONCLUSION: The method has low detection limit, good precision and high sensitivity, which is suitable for the determination of arsenics in human urine.
