Toxic effect of nano-cerium oxide on zebrafish embryonic early development
10.11763/j.issn.2095-2619.2020.01.008
- Author:
Yu CHEN
1
;
Yan FAN
1
;
Fan SHU
;
Wei ZHU
;
Wei XIE
1
;
Rengui BADE
1
;
Shuyuan JIANG
1
;
Xiaolei LIU
1
;
Guo SHAO
1
;
Gang WU
1
;
Xiaoe JIA
1
Author Information
1. Biomedicine Research Center, Neuroscience institute, Baotou Medical College Baotou, Inner Mongolia 014040, China
- Publication Type:Journal Article
- Keywords:
Nanometer;
Cerium oxide;
Zebrafish;
Apoptosis;
Autophagy;
Toxic
- From:
China Occupational Medicine
2020;47(01):48-52
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To study the effect of nano-cerium oxide on the early development of zebrafish embryos. METHODS: The well-developed zebrafish embryos were randomly divided into the control group and the 50, 100, 200, 400 and 800 mg/L dose groups, with 40 embryos in each group. The dose groups were treated with nano-cerium oxide at the corresponding mass concentration for 5 days. The control group received no treatment. The death and malformation of embryos were observed. The heart rate of zebrafish embryos was recorded using confocol microscope. The protein expression of microtubule-associated protein 1 light chain 3(LC3) and cleaved Caspase-3 and were observed by Western blot technology. RESULTS: The death and embryonic malformation rate of zebrafish embryos increased with the increase of doses, showing statistical significance(P<0.01). The heart rate of the 800 mg/L dose group was decreased compared with the control group [(77±8) vs(93±4) beats/min, P<0.01]. There was no statistical significant difference in LC3-Ⅱ protein expression in each groups(P>0.05). The cleaved Caspase-3 protein expression increased in all dose groups compared with the control group(P<0.05). The cleaved Caspase-3 protein expression in the 200 mg/L dose group was higher than that in the 50 mg/L dose group(P<0.05). CONCLUSION: The nano-cerium oxide may induce cell apoptosis, causing toxic effect in early development of zebrafish embryos.
