Effects of hyperthermia on PANDAR, LncRNA-p21 and ST8SIA gene in A549 Cell
10.11763/j.issn.2095-2619.2019.04.009
- Author:
Zhiqiang LI
1
;
Ruobing ZHANG
1
;
Yuefeng HE
1
;
Chang LIU
1
;
Xinan WU
1
;
Li SHANG
1
;
Nina PING
1
;
Yuan ZHANG
Author Information
1. The Public Health Institute of Kunming Medical University Kunming, Yunnan 650500, China
- Publication Type:Journal Article
- Keywords:
Hyperthermia;
A549 cell;
PANDAR;
LncRNA-p21;
ST8SIA
- From:
China Occupational Medicine
2019;46(04):446-448
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To investigate the effect of hyperthermia on the expression of PANDAR, LncRNA-p21 and ST8SIA genes in the human lung adenocarcinoma A549 cells. METHODS: A549 cells were randomly divided into 4 groups. The A549 cells in control group were cultured at 37 ℃; the cells at experimental groups were cultured at 40, 42 or 44 ℃ respectively. The cells in these 4 groups were incubated for 1 hour, and the levels of PANDAR, LncRNA-p21 and ST8SIA genes were analyzed by real-time fluorescence quantitative polymerase chain reaction. RESULTS: In cells cultured at 40, 42 or 44 ℃ experimental groups, the relative expression of PANDAR gene was lower than that of control group(P<0.05). In cells cultured at 44 ℃ experimental group, the relative expression of PANDAR gene was lower than that of the 40 and 42 ℃ experimental groups(P<0.05). There was no significant change in the relative expression of LncRNA-p21 and ST8SIA genes among the four groups(P>0.05). CONCLUSION: Hyperthermia decrease the expression of PANDAR gene in A549 cells.
