Detecting three halogenated hydrocarbons in blood by gas chromatography-mass spectrometry
10.11763/j.issn.2095-2619.2019.03.021
- Author:
Xiaoxuan CAI
1
;
Weifeng RONG
;
Banghua WU
;
Aihua ZHANG
;
Xinguang ZHONG
1
;
Jianmei PENG
1
;
Lihe YE
1
;
Zheng MA
1
;
Si TANG
1
Author Information
1. Dongguan Prevention and Treatment Center for Occupational Disease Dongguan,Guangdong 523008,China
- Publication Type:Journal Article
- Keywords:
Gas chromatography-mass spectrometry;
Blood sample;
Headspace analysis;
Halogenated hydrocarbon;
Dichloromethane;
Trichloromethane;
1,2-Dichloroethane
- From:
China Occupational Medicine
2019;46(03):363-370
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish a method for detecting dichloromethane,trichloromethane and 1,2-dichloroethane in blood by gas chromatography-mass spectrometry. METHODS: Using static headspace analysis, three halogenated hydrocarbons in blood samples were separated by DB-5 MS elastic capillary column and detected by gas chromatographymass spectrometry. RESULTS: There was a good linear relationship in the selected range of dichloromethane,trichloromethane and 1,2-dichloroethane in blood. The linear correlation coefficient was greater than 0. 999 8. The detection limit and the lower limit of quantitation was 0. 19-0. 28 and 0. 64-0. 93 μg/L,respectively. The average recovery rate was 95. 1%-106. 6%. The within-run and between-run relative standard deviation was 2. 9%-4. 9% and 5. 0%-7. 0%,respectively. The samples could be preserved at room temperature or 4 ℃ for 3 days and at-8 ℃ or below for7 days. CONCLUSION: With the features of high sensitivity,precision,accuracy,easy operation and less interference,this method is suitable for detecting dichloromethane,trichloromethane and 1,2-dichloroethane in the blood.
