The effects of NOX1 and NF-κB on TNF-α induced oxidative damage in A549 cells
10.11763/j.issn.2095-2619.2019.02.010
- Author:
Fang ZHOU
1
;
Ya'nan REN
1
;
Na WANG
1
;
Ruonan ZHAI
1
;
Yanqiu XIA
1
;
Teng XUE
1
;
Kai ZHANG
1
;
Wu YAO
1
Author Information
1. School of Public Health, Zhengzhou University Zhengzhou, Henan 450001, China
- Publication Type:Journal Article
- Keywords:
A549 cells;
Nicotinamide adenine dinucleoside phosphate oxidases 1;
Nuclear factor-kappa B;
Tumor necrosis factor-alpha;
Reactive oxygen species;
Oxidative damage
- From:
China Occupational Medicine
2019;46(02):188-193
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To investigate the effects of nicotinamide adenine dinucleoside phosphate oxidases 1(NOX1) and nuclear factor-kappa B(NF-κB) in tumor necrosis factor-α(TNF-α)-induced oxidative damage in A549 cells. METHODS: i) TNF-α was used to stimulated A549 cells at the concentrations of 0.0, 10.0, 25.0 and 50.0 μg/L. Cell inhibition rate in each group was tested by CCK-8 assay to select the appropriate concentration. ii) A549 cells in logarithmic growth phase were divided into blank control group, solvent control group, TNF-α group, diphenylene iodine(DPI) group and TNF-α+DPI group for NOX1 inhibitor experiment. Logarithmic growth phase A549 cells were divided into blank control group, TNF-α group, BAY11-7082 group and TNF-α+BAY11-7082 group for NF-κB inhibitor experiment. The relative expression of NOX1 and p65 protein in each group was detected by Western blot method. The relative expression of intracellular reactive oxygen species(ROS) were detected by flow cytometry. RESULTS: i) The inhibition rate of A549 cells increased with the increase of TNF-α dose(P<0.05), and 25.0 μg/L was selected as the stimulation dose of TNF-α in subsequent experiments. ii) The relative expression of NOX1, p65 protein and ROS in the TNF-α group was higher than that in the blank control group, solvent control group and DPI group, respectively(P<0.05). The above indexes in TNF-α+DPI group were lower than that in TNF-α group(P<0.05), but higher than that in DPI group(P<0.05). The relative expression of NOX1, p65 protein and ROS in the TNF-α group were higher than that in the blank control group and the BAY11-7082 group(P<0.05), while the above indicators in the TNF-α+BAY11-7082 group were lower than that in the TNF-α group(P<0.05). CONCLUSION: Inhibition of NOX1 or NF-κB can alleviate the oxidative damage induce by TNF-α in A549 cells.
