Acute 1,2-dichloroethane exposure induced differential gene expression of liver phaseⅠmetabolic enzymes in SD rats

Xiao Yin; Hao Cheng; Yizhou Zhong; Jiewei Zheng; Zhiwei Xie; Lihai Zeng; Weifeng Rong; Fengrong LU; Manqi Huang; Guoliang LI; Boxuan Liang; Liang Jiang; Li Lin; Jianxun Huang; Zhenlie Huang

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Acute 1,2-dichloroethane exposure induced differential gene expression of liver phaseⅠmetabolic enzymes in SD rats

Author: Xiao YIN ^{1
,

2} ; Hao CHENG ; Yizhou ZHONG ^{1
,

2} ; Jiewei ZHENG ^{1
,

2} ; Zhiwei XIE ^{1
,

2} ; Lihai ZENG ^{1
,

2} ; Weifeng RONG ^{1
,

2} ; Fengrong LU ^{1
,

2} ; Manqi HUANG ^{1
,

2} ; Guoliang LI ^{1
,

2} ; Boxuan LIANG ^{1
,

2} ; Liang JIANG ; Li LIN ; Jianxun HUANG ^{1
,

2} ; Zhenlie HUANG ^{1
,

2}
Author Information

1. Guangdong Provincial Hospital for Occupational Disease Prevention and Treatment
2. Guangdong Provincial Key Laboratory of Occupational Disease Prevention and Treatment Guangzhou, Guangdong 510300, China
Publication Type:Journal Article
Keywords: 1,2-Dichloroethane; Inhalation; Liver; Metabolic enzyme; Differential expression; Rat
From: China Occupational Medicine 2019;46(02):163-166
CountryChina
Language:Chinese
Abstract: OBJECTIVE: To investigate the effect of 1,2-dichloroethane(1,2-DCE) acute inhalation exposure on the differential gene expression of phase Ⅰ metabolic enzymes. METHODS: The specific pathogen free SD rats were randomly divided into control group(16 rats), low-and high-dose groups(24 rats in each group, half males and half females). Low-and high-dose group were given daily 600, 1 800 mg/m~(3 ) of 1,2-DCE, and the control group given the fresh air by dynamic inhalation for 8 hours per day for consecutive 7 days. After the end of exposure, the relative mRNA expression of cytochrome P450 2 E1(CYP2 E1), alcohol dehydrogenase(ADH1) and acetaldehyde dehydrogenase 3 alpha 1(ALDH3α1) in the liver tissue was detected by real-time fluorescence quantitative polymerase chain reaction. RESULTS: The relative expression of CYP2 E1 in male high-dose group was higher than that in male low-dose group and female high-dose group(P<0.05). The relative expression of ADH1 in male low-and high-dose groups was higher than that in male control group(P<0.05). The relative expression of ADH1 in male high-dose group was higher than that in male low-dose group and female high-dose group(P<0.05). The relative expression of ALDH3α1 in high-dose group was higher than that in control group and low-dose group(P<0.05). CONCLUSION: High dose 1,2-DCE could increase the gene expression of phase Ⅰ metabolic enzymes in rat liver. The 1,2-DCE has more obvious effect in male rats than in female rats.