1,2-Dichloroethane-induced apoptosis in human astrocytes through mitochondrial pathway

Kengkeng Chen; Guanchao Lai; Lihai Zeng; Boxuan Liang; Yizhou Zhong; Nianguang Chen; Manqi Huang; Jiewei Zheng; Liang Jiang; Li Lin; Jun Liu; Junying Jiang; Qianling Zheng; Zhenlie Huang

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1,2-Dichloroethane-induced apoptosis in human astrocytes through mitochondrial pathway

Author: Kengkeng CHEN ^{1
,

2} ; Guanchao LAI ^{1
,

2} ; Lihai ZENG ^{1
,

2} ; Boxuan LIANG ^{1
,

2} ; Yizhou ZHONG ^{1
,

2} ; Nianguang CHEN ^{1
,

2} ; Manqi HUANG ^{1
,

2} ; Jiewei ZHENG ^{1
,

2} ; Liang JIANG ; Li LIN ; Jun LIU ; Junying JIANG ; Qianling ZHENG ^{1
,

2} ; Zhenlie HUANG ^{1
,

2}
Author Information

1. Guangdong Provincial Hospital for Occupational Disease Prevention and Treatment
2. Guangdong Provincial Key Laboratory of Occupational Disease Prevention and Treatment Guangzhou,Guangdong 510300,China
Publication Type:Journal Article
Keywords: 1,2-Dichloroethane; Human astrocyte; Apoptosis; Mitochondrial pathway; Differentially expressed; Protein chip
From: China Occupational Medicine 2018;45(04):417-423
CountryChina
Language:Chinese
Abstract: OBJECTIVE: To explore the molecular mechanism underlying 1,2-dichloroethane(1,2-DCE) induced apoptosis by screening differentially expressed proteins in human astrocytes( HAs). METHODS: HAs were cultured in complete medium with 1,2-DCE at various concentrations of 0-80 or 0-40 mmol/L. After 24 hours,apoptosis of HAs was evaluated using flow cytometry and staining with annexin Ⅴ-fluoresce in isothiocyanate and propidium iodide. An AAH-APO-1-2 protein chip was used to screen differentially expressed proteins and quantitative real-time polymease chain reaction(qRT-PCR) was used to verify related differentially expressed genes(DEGs). RESULTS: At 1,2-DCE concentrations of0-80 mmol/L,the total apoptosis rate of HAs increased with 1,2-DCE concentrations in a dose-dependent manner( P <0. 01). Seven different kinds of proteins were screened out by apoptotic protein chip. Among them,the expression of insulin-like growth factor-binding protein( IGFBP)-1,IGFBP-4 and cytochrome C( Cyto C) were up-regulated,while the expression of P27,cysteine aspartic acid specific protease-3( Caspase-3),B-cell lymphoma-2 interacting mediator of cell death( BIM) and BH3 interacting domain death agonist( BID) were down-regulated compared with the control group. The result of DEGs verified by qRT-PCR showed that the expression of mRNA of IGFBP-1,IGFBP-4 and Cyto C at 1,2-DCE concentrations of 40 mmol/L was up-regulated. This result was in consistent with the trend of target expression in the protein chip. The mRNA expression of Caspase-3,BIM and BID was also up-regulated. CONCLUSION: 1,2-DCE induces apoptosis of HAs through mitochondrial pathway.