Detecting hexamethylene diamine in workplace air by high performance liquid chromatography
10.11763/j.issn.2095-2619.2018.03.023
- Author:
Jue WANG
1
;
Xiaolin RUAN
;
Yanrong GUO
1
;
Ding SHEN
1
;
Neng ZHOU
1
Author Information
1. Occupation Disease Prevention and Treatment Hospital,China Pingmei Shenma Group Pingdingshan,Henan 467099,China
- Publication Type:Journal Article
- Keywords:
High performance liquid chromatography;
Hexamethylene diamine;
Derivative;
Workplace;
Air
- From:
China Occupational Medicine
2018;45(03):377-380
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish a method for detecting hexamethylene diamine in workplace air by high performance liquid chromatography( HPLC).METHODS: Hexamethylene diamine in the workplace air was collected by silica gel tube,and each was added with a concentration of 0.05 mol/L sodium bicarbonate solution,each with 0.60 mL of dansyl chloride solution( a mass concentration of 240.00 mg/L),and the volume was adjusted to 5.00 mL with acetonitrile,heating for 40.00 min in bath water,acetonitrile:water( 75:25,V/V) as the mobile phase,quantitated by the standard curve method,using HPLC for determinationstandard.RESULTS: The linear range of hexamethylene diamine was 0.040 0-6.000 0 mg/L,the correlation coefficient was 0.999 3,and the detection limit was 0.003 8 mg/L,and the minimum detection concentration was 0.002 5 mg/m~3( calculated by sample volume of 3.0 L); The within-run relative standard deviation( RSD) was 2.1%-3.0%,and the between-run RSD was 2.9%-3.6%.The average desorption efficiency of the method was 91.4%-94.1%.The sampling efficiency was 98.5%-99.6%.CONCLUSION: The method is simple,rapid,sensitive,accurate and suitable for the detecting hexamethylene diamine concentration in workplace air.
