Effect of different anesthetics and blood sampling methods on blood routine test in SD rats and Kunming mice

Jiewei Zheng; Fengrong LU; Guoliang LI; Zhiwei Xie; Yizhou Zhong; Lihai Zeng; Manqi Huang; Yating Zhang; Xiao Yin; Yuli Zeng; Chen Gao; Haiyan Zhang; Jiejiao WU; Liang Jiang; Zhenlie Huang

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Effect of different anesthetics and blood sampling methods on blood routine test in SD rats and Kunming mice

Author: Jiewei ZHENG ^{1
,

2} ; Fengrong LU ^{1
,

2} ; Guoliang LI ^{1
,

2} ; Zhiwei XIE ^{1
,

2} ; Yizhou ZHONG ^{1
,

2} ; Lihai ZENG ^{1
,

2} ; Manqi HUANG ^{1
,

2} ; Yating ZHANG ^{1
,

2} ; Xiao YIN ^{1
,

2} ; Yuli ZENG ^{1
,

2} ; Chen GAO ; Haiyan ZHANG ; Jiejiao WU ; Liang JIANG ; Zhenlie HUANG ^{1
,

2}
Author Information

1. Institute of Toxicology,Guangdong Province Hospital for Occupational Disease Prevention and Treatment
2. Guangdong Provincial Key Laboratory of Occupational Disease Prevention and Treatment Guangzhou,Guangdong 510300,China
Publication Type:Journal Article
Keywords: Anesthetic; Blood sampling method; Blood routine; Ether; Chloral hydrate; Pentobarbital sodium; Experimental animal
From: China Occupational Medicine 2018;45(01):51-54
CountryChina
Language:Chinese
Abstract: OBJECTIVE: To compare the effects of different anesthetics and blood sampling methods on blood routine test results in experimental animals. METHODS: A total of 42 specific pathogen free( SPF) male Sprague Dawley( SD) rats and 59 SPF male Kunming( KM) mice were randomly divided into 4 groups( control group,ether group,chloral hydrate group and pentobarbital sodium group). Ether group animals were treated with ether inhalation anesthesia; animals in chloral hydrate group and pentobarbital sodium group were injected intraperitoneally with chloral hydrate or pentobarbital sodium. The control group received no anesthesia treatment. Blood samples were collected by different ways: orbital venous plexus,abdominal aorta or eyeball enucleation. White blood cell( WBC) count,red blood cell( RBC) count,platelet(PLT) count,hemoglobin(Hb) level and hematocrit(HCT) in blood samples were analyzed. RESULTS: The RBC count,Hb level and HCT of SD rats in pentobarbital sodium group were significantly lower than those in control group( P <0. 05). The HCT of SD rats in ether group was lower than that in control group( P < 0. 05). The WBC count of orbital venous plexus of KM mice was lower than that taken by eyeball enucleation in control group( P < 0. 05),but the WBC count of orbital venous plexus was higher than that taken by eyeball enucleation in chloral hydrate group( P < 0. 05). The RBC count,Hb level,HCT of KM mice in pentobarbital sodium group were significantly lower than those in control group(P < 0. 05). CONCLUSION: The anesthetic can affect the blood routine test results of experimental animals. Different blood sampling methods have effects on blood routine test results of KM mice.