Relationship of PAHs metabolites,DNA oxidative damage and RING2 expression in coke oven workers
10.11763/j.issn.2095-2619.2018.01.002
- Author:
Yanli LIU
1
;
Hongjie ZHANG
1
;
Baolong PAN
1
;
Huitao ZHANG
1
;
Yingying NIU
1
;
Ye FU
1
;
Jin YANG
1
Author Information
1. School of Pubilc Health,Shanxi Medical University Taiyuan,Shanxi 030001,China
- Publication Type:Journal Article
- Keywords:
Polycyclic aromatic hydrocarbons;
Metabolite;
DNA oxidative damage;
1-Hydroxypyrene;
8-Hydroxy deoxyguanosine;
Ring finger protein 2
- From:
China Occupational Medicine
2018;45(01):7-11
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To investigate the relationship of polycyclic aromatic hydrocarbons( PAH) metabolites,DNA oxidative damage and ring finger protein 2( RING2) expression in coke oven workers. METHODS: A judgment sampling method was used to select 497 coke oven workers in a steel plant as exposure group and 175 water treatment workers in the same plant as control group. The levels of urinary 1-hydroxypyrene, 2-hydroxynathalene, 2-hydroxyfluorene,9-hydroxyphenanthrene and 8-hydroxy deoxyguanosine(8-OHd G) were detected by high performance liquid chromatography.The RING2 expression in whole blood was measured by reverse transcription-polymerase chain reaction. RESULTS: The relative expression of urinary 1-hydroxypyrene,2-hydroxynathalene,2-hydroxyfluorene,9-hydroxyphenanthrene and RING2 in exposure group were higher than that in control group( P < 0. 01). The logistic regression analysis indicated that the higher the level of 1-hydroxypyrene,the higher the risk of high-RING2 expression( P < 0. 05) after adjusting for factors such as sex,age,smoking status,alcohol drinking,2-hydroxynathalene,2-hydroxyfluorene and 9-hydroxyphenanthrene.In 1-hydroxypyrene middle and high level groups,the 8-OHd G concentration of high-RING2 expression workers was significantly higher than those of low-RING2 expression workers( P < 0. 05). CONCLUSION: With the increase of urinary1-hydroxypyrene,the risk of high-RING2 expression was elevated,the degree of DNA oxidative damage was gradually increased.
