Effect of 1,2-dichloroethane on learning and memory in NIH mice
10.11763/j.issn.2095-2619.2018.01.001
- Author:
Yating ZHANG
1
;
Yizhou ZHONG
;
Guoliang LI
;
Jiewei ZHENG
;
Jiejiao WU
;
Lihai ZENG
;
Manqi HUANG
;
Zhiwei XIE
;
Fengrong LU
;
Boxuan LIANG
;
Liang JIANG
;
Qianling ZHENG
;
Zhenlie HUANG
1
Author Information
1. School of Public Health,Guangdong Pharmaceutical University Guangzhou,Guangdong 510315,China
- Publication Type:Journal Article
- Keywords:
1,2-Dichloroethane;
Subacute;
Dynamic inhalation;
Morris water maze test;
Learning and memory;
Neurobehavioral;
Mice
- From:
China Occupational Medicine
2018;45(01):1-6
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To investigate the effects of subacute systemic inhalation exposure of 1,2-dichloroethane(1,2-DCE) on learning and memory in NIH mice. METHODS: Forty-five specific pathogen free healthy 7-week-old NIH mice were randomly divided into control,low-dose and high-dose groups with 5 female mice and 10 male mice in each group. The mice were exposed to 1,2-DCE at dosages of 0. 00,100. 00 and 350. 00 mg/m3 for 6 hours per day for consecutive 28 days by dynamic systemic inhalation. The neurobehavioral tests of mice were performed before and after the first to fourth weeks of exposure using the Morris water maze test. RESULTS: There was no significant difference in body weight and swimming speed among the three groups of mice( P > 0. 05). The navigation experiment results showed that the escape latency of mice in both low-and high-dose groups were longer than that of the control group at the same time point(P < 0. 05) during 1-4 weeks after exposure. In the control group,the escape latency was shorter than that of the same group before exposure( P < 0. 05). The escape latency of high-dose group prolonged with the increase of exposure time,and in the 4 th week the escape latency was significantly higher than that of the same group before exposure( P < 0. 05).The experiment results of space exploration indicated that the first time of crossing platform in low-and high-dose groups were longer than that of the control group at the second to the fourth week( P < 0. 05). The target quadrant retention time and the number of crossing the platform in the low-and high-dose groups were lower than those in the control group( P <0. 05). CONCLUSION: Subacute inhalation exposure of 1,2-DCE can impair the learning and memory ability of NIH mice.The high-dose exposure may reduce learning ability in mice in a time-effect manner.
