Repair effects of bone marrow mesenchymal stem cells on benzene-induced hematopoietic injury in mice
10.11763/j.issn.2095-2619.2017.05.001
- Author:
Na ZHAO
1
,
2
;
Xiangrong SONG
1
,
2
;
Qiuying LIU
1
,
2
;
Hongling LI
1
,
2
;
Xuemin CAI
;
Hailan WANG
1
,
2
Author Information
1. Guangdong Province Hospital for Occupational Disease Prevention and Treatment
2. Guangdong Provincial Key Laboratory of Occupational Disease Prevention and Treatment Guangzhou,Guangdong 510300,China
- Publication Type:Journal Article
- Keywords:
Bone marrow;
Mesenchymal stem cells;
Benzene;
Hematopoietic injury;
Transplantation;
Repair effect;
Interferon-γ
- From:
China Occupational Medicine
2017;44(05):537-541
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To explore the repair effects of bone marrow mesenchymal stem cells( BMSCs) on hematopoietic injury induced by benzene poisoning in mice. METHODS: Five specific pathogen free healthy male Kunming mice were selected to obtain BMSCs through bone marrow attachment culturing method. The Kunming mice were randomly divided into poisoning group and BMSCs transplantation group,18 mice in each group,after the benzene poisoning model was established by subcutaneous multi-point injection of benzene and oil mixture 3 times/week,10 weeks continuously. Each group was injected through tail vein with 250. 0 μL 0. 9% sodium chloride solution or 250. 0 μL BMSCs suspension( cell density 2 × 109/L) once per week for 4 weeks,respectively. The control group( 10 mice) was not given any treatment.Mice were euthanized 2 weeks after treatment. The blood routine examination was conducted. Nucleated cells in bone marrow were observed after Giemsa staining. The clones of hemopoietic progenitor cells were counted and the levels of serum interferon-γ( IFN-γ) were examined using enzyme-linked immune sorbent assay. RESULTS: The mouse model of chronic benzene poisoning was established successfully. After the BMSCs transplantation treatment,the white blood cell count,platelet count,red blood cell count,hemoglobin level and bone marrow nucleated cell as well as granulocyte-macrophage colony forming unit( CFU-GM) in benzene poisoning group were significantly decreased compared with control group( P <0. 01),while those indexes of BMSCs treatment group were higher than that of benzene poisoning group( P < 0. 05). The counts of platelet,red blood cell,bone marrow nucleated cell and CFU-GM in BMSCs treatment group were significantly lower than that of control group( P < 0. 05). The level of serum IFN-γ in benzene poisoning group was higher than that of control group( P < 0. 01),and serum IFN-γ level in BMSCs treatment group was lower than that of benzene poisoning group( P < 0. 01). There was no significant difference of IFN-γ level in BMSCs treatment group compared with control group( P > 0. 05). CONCLUSION: BMSCs have repair effects on hematopoietic system injury caused by benzene poisoning.
