Effects of different radiation on chromosome aberration in human lymphoblastoid cells
10.11763/j.issn.2095-2619.2017.03.019
- Author:
Ruifeng ZHANG
1
;
Yayi YUAN
1
;
Yue REN
1
;
Zhongxin ZHANG
1
;
Juancong DONG
1
;
Xuhong DANG
1
;
Lihong XING
1
;
Yahui ZUO
1
;
Zhikai DUAN
1
Author Information
1. China Institute for Radiation Protection Taiyuan,Shanxi 030006,China
- Publication Type:Journal Article
- Keywords:
~(56)Fe~(17+) ion beam;
~(12)C~(6+) ion beam;
~(60)Co γ ray;
Human lymphoblastoid cell;
Chromosome aberration;
Dicentric;
Centric ring
- From:
China Occupational Medicine
2017;44(03):341-344
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To compare the effects of ~(56)Fe~(17+),~(12)C~(6+)ion beams and~(60)Co γ rays on chromosome aberration in human lymphoblastoid cells. METHODS: The human lymphoblastoid cells were divided into 0. 1,0. 3,0. 5,0. 7,1. 0,2. 0 Gy irradiated groups and 0. 0 Gy control group. They were separately exposed to ~(56)Fe~(17+)ion beams( linear energy transfer was 400. 0 ke V/μm),~(12)C~(6+)ion beams( linear energy transfer was 26. 0 ke V/μm) and~(60)C γ rays. Chromosome specimens were harvested 48 hours after irradiation. The effects of different radiation on dicentric plus centric ring( “d + r”) aberration rate and chromosome aberration in human lymphoblastoid cells were detected by light microscope with artificial counting. RESULTS: The “d + r”aberration rates induced by 0. 3-2. 0 Gy ~(12)C~(6+)ion beams were significantly higher than those of ~(56)Fe~(17+)ion beams and~(60)Co γ rays at the same dose( P < 0. 017). Chromosome aberration cell rates of 0. 1-2. 0 Gy ~(12)C~(6+)ion beams were significantly higher than those of ~(56)Fe~(17+)ion beams and~(60)C γ rays at the same dose( P < 0. 017). At the dose range of 0. 0-2. 0 Gy,chromosome aberration effects of three kinds of radiations were gradually increased( P < 0. 01). The relative biological effectiveness of ~(56)Fe~(17+)ion beams was lower than that of ~(12)C~(6+)ion beams.CONCLUSION: The chromosome aberration induced by ~(12)C~(6+)ion beams was more serious than that of~(60)Co γ rays and ~(56)Fe~(17+)ion beams.
