Mechanism of coke oven emissions-induced acute toxicity in HL-60 cells
10.11763/j.issn.2095-2619.2017.01.003
- Author:
Meili SHEN
1
;
Qiuyan XIE
1
;
Hongli LI
1
;
Yanhua WANG
1
;
Qianpeng JI
1
;
Qingrong WANG
1
;
Yufei DAI
1
;
Huawei DUAN
1
Author Information
1. Key Laboratory of Chemical Safety and Health,National Institute for Occupational Health and Poison Control,Chinese Center for Disease Control and Prevention Beijing 100050,China
- Publication Type:Journal Article
- Keywords:
Coke oven emissions;
HL-60 cells;
Reactive oxygen species;
Nuclear factor-κB
- From:
China Occupational Medicine
2017;44(01):14-19
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish the cell model using human leukemia cell line HL-60 for exposure of coke oven emissions( COE) in vitro and to explore the mechanism of COE-induced acute toxicity in HL-60 cells. METHODS: HL-60 cells were collected in their logarithmic growth phase and cultured in medium that had final concentrations of COE in 2. 5,5. 0,10. 0 and 20. 0 mg / L for 24 hours. Cell survival rate was examined by CCK-8 assay. The cytotoxicity was evaluated using lactate dehydrogenase release assay. Reactive oxygen species( ROS) production was determined by the 2',7'-dichlorofluorescein diacetate and nitroblue tetrazolium method. The activation of nuclear factor-κB( NF-κB) pathway was evaluated by western blot. RESULTS: With the increasing exposure concentrations of COE,the cytotoxicity of HL-60 cells increased( P < 0. 01),the cell survival rate decreased( P < 0. 01),intracellular ROS decreased( P < 0. 01),whereas extracellular ROS increased( P < 0. 01). These changes had a dose-effect relationship. The levels of phospho-nuclear factor-kappa B p65 and phospho-inhibitor of kappa Bα were higher in all the COE-treated cells compared with untreated cells( P < 0. 05),with no dose-effect relationship. CONCLUSION: COE could cause acute toxicity in HL-60 cells in a doseeffect relationship. The mechanism may be related to the COE-induced in-balanced ROS release and removal,leading to the activation of NF-κB pathway. HL-60 cells can be used as a common cell line for COE hematotoxicity analysis.
