The intestinal absorption characteristics of five active components in Lizhong Decoction
10.16438/j.0513-4870.2021-0097
- VernacularTitle:理中汤提取物中5种主要活性成分在体肠吸收特征研究
- Author:
Yue WAN
;
Yu-meng SHEN
;
Jun-feng ZOU
;
Meng-jun CHEN
;
Zhi-miao ZHANG
;
Shu JIANG
;
Da-wei QIAN
;
Jin-ao DUAN
- Publication Type:Research Article
- Keywords:
Lizhong decoction;
UPLC-TQ-MS;
italic>in situ intestinal perfusion;
intestinal absorption characteristics;
gravimetric method
- From:
Acta Pharmaceutica Sinica
2021;56(6):1689-1695
- CountryChina
- Language:Chinese
-
Abstract:
The intestinal absorption properties of the main effective components (glycyrrhizic acid, isoliquiritigenin, 6-gingerol, ginsenoside Rb1, atractylode-I) in Lizhong decoction (LZD) extracts were investigated with an in situ single-pass intestinal perfusion model in rats. UPLC-TQ-MS was used to determine the concentration of the five components in the intestinal perfusion. Animal welfare and experimental procedures were in accordance with the regulations of the Animal Ethics Committee of Nanjing University of Chinese Medicine. As evaluation indexes for the intestinal absorption characteristics, the absorption rate constant (Ka) and the apparent permeability coefficient (Peff) of the five main ingredients were analyzed. Results showed that the best absorption sites for glycyrrhizic acid, isoliquiritin and 6-gingerol were the ileum, colon and duodenum, respectively, and the differences between different intestinal segments were statistically significant (P <0.05). There was no notable difference in Ka and Peff between ginsenoside Rb1 and atractylode-I in the different intestinal segments (P > 0.05), suggesting that they were absorbed throughout. The five components were well-absorbed in the whole intestine (Peff > 1.0×10-3 cm·min-1), indicating that LZD is suitable for preparing sustained, controlled release and enteric-coated preparations.
