Epidemiological characterization of sapovirus gastroenteritis aggregation in a kindergarten in Jinshan District， Shanghai
10.19428/j.cnki.sjpm.2021.19643
- VernacularTitle:上海市某幼儿园一起札如病毒引发的胃肠炎疫情流行分析
- Author:
Lin ZHU
1
;
Zhao-peng DONG
1
;
Jian-ru DU
1
;
Ping-hua MO
1
;
Tang WANG
1
;
Can-lei SONG
1
Author Information
1. Jinshan Center for Disease Control and Prevention， Shanghai 201599， China
- Publication Type:Research Article
- Keywords:
sapovirus;
acute gastroenteritis;
outbreak;
epidemiology
- From:
Shanghai Journal of Preventive Medicine
2021;33(6):521-524
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the epidemiological characteristics of an aggregational gastroenteritis and determine the genotypes of sapovirus， and to provide scientific basis for formulating effective control strategies. Methods:Unified case definition， active case search and descriptive epidemiology were used to analyze the epidemic. Feces or anal swabs of untreated students， teachers， canteen staff as well as canteen environment samples were collected. Norovirus and sapovirus nucleic acid tests were conducted by real-time fluorescent RT-PCR， and sapovirus nucleic acid was amplified by conventional RT-PCR. The gene region of capsid protein was analyzed by MEGA7.0 software and phylogenetic tree was constructed. Results:A total of 12 cases were reported in the epidemic， and the incidence rate was 44.44%. All reported cases， with vomiting symptoms， were found in the same class. The epidemic showed a point-based outbreak. The first case became the source of infection in class， and the epidemic lasted for 8 days. Real-time fluorescent RT-PCR assay confirmed that five children's feces were positive for sapovirus nucleic acid， and the first-episode children's feces were positive for sapovirus and GII norovirus nucleic acid. Sequence alignment result showed that the sapovirus strains belonged to GI.1 type with homologous genes. Conclusion:Based on the clinical manifestations， field epidemiological investigation and laboratory test results， we confirm that the first case of the epidemic in class is caused by GI.1 sapovirus infection. The epidemic is effectively controlled by comprehensive measures such as case isolation and disinfection.
